Raut Sangram L, Rich Ryan, Fudala Rafal, Kokate R, Kimball J D, Borejdo Julian, Vishwanatha Jamboor K, Gryczynski Zygmunt, Gryczynski Ignacy
Center for Fluorescence Technologies and Nanomedicine, UNT Health Science Center, Fort Worth, TX, 76107l, USA.
Curr Pharm Biotechnol. 2014;14(13):1139-44. doi: 10.2174/1389201015666140523161038.
Although BSA Au clusters fluoresce in red region (λmax: 650 nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1 µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.
尽管牛血清白蛋白金簇在红色区域（λmax：650 nm）发出荧光，但由于其低荧光量子产率（约6%），其用途有限。在此我们报告了一种使用多脉冲激发方案的荧光生物纳米探针牛血清白蛋白金簇的增强荧光成像应用。多脉冲激发利用了牛血清白蛋白金簇的长荧光寿命（>1 µs），并使其荧光强度比短寿命的细胞自发荧光增强了15倍。此外，我们还表明，通过使用时间门控检测策略，信号（牛血清白蛋白金簇的荧光）与噪声（自发荧光）的比率可以提高30倍。因此，利用多脉冲激发，长寿命探针可用于开发生化分析并实现零背景的光学成像。
