Raut S L, Fudala R, Rich R, Kokate R A, Chib R, Gryczynski Z, Gryczynski I
Center for Commercialization of Fluorescence Technologies, Department of Cell Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, USA.
Nanoscale. 2014 Mar 7;6(5):2594-7. doi: 10.1039/c3nr05692a. Epub 2014 Jan 27.
The work presented here reports the use of long lifetime (>1 μs) BSA Au clusters as a cellular/tissue, time gated, intensity imaging probe. By collecting the emission signal 50 ns post excitation, one can off-gate the intense auto-fluorescence background, thereby greatly enhancing the clarity/specificity in fluorescence imaging.
本文所展示的工作报道了使用长寿命（>1微秒）的牛血清白蛋白金簇作为细胞/组织的时间门控强度成像探针。通过在激发后50纳秒收集发射信号，可以去除强烈的自发荧光背景，从而大大提高荧光成像的清晰度/特异性。
