Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Science, Hainan University, Haikou 570228, PR China.
Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Science, Hainan University, Haikou 570228, PR China; State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China.
J Hazard Mater. 2021 Aug 15;416:125759. doi: 10.1016/j.jhazmat.2021.125759. Epub 2021 Mar 26.
Fluorescent probes with in-situ visual feature have received numerous attentions for detecting doxycycline (DC), a semisynthetic tetracycline antibiotic widely used in animal husbandry. However, reported fluorescent probes commonly fail to selectively detect DC among tetracycline antibiotics due to their structural similarity. In this work, bovine serum albumin-capped gold nanoclusters (BSA-AuNCs) were ingeniously used as the ratiometric fluorescent probe for detecting DC over other tetracycline antibiotics through the selective sensitization effect of BSA on DC. After adding DC, the red fluorescence of BSA-AuNCs almost remained unchanged, while the green fluorescence of DC also emerged under the sensitization of BSA. BSA-AuNCs showed the highest response toward DC among tetracycline antibiotics ascribed to the strongest sensitization effect of BSA on DC. BSA-AuNCs also displayed the features of simple synthesis, short response time (1 min) and low detection limit (36 nM). BSA-AuNCs were finally applied to detecting DC in fish samples, and further fabricated into test strips for ease of carrying. Thus, this work proposes an efficient strategy to design fluorescent probe for selectively detecting DC among tetracycline antibiotics.
具有原位可视化特征的荧光探针因其在动物养殖中广泛应用的半合成四环素抗生素强力霉素 (DC) 的检测而受到广泛关注。然而，由于结构相似，报道的荧光探针通常无法在四环素抗生素中选择性地检测 DC。在这项工作中，牛血清白蛋白包覆的金纳米团簇 (BSA-AuNCs) 被巧妙地用作比率荧光探针，通过 BSA 对 DC 的选择性敏化作用来检测 DC 以外的其他四环素抗生素。加入 DC 后，BSA-AuNCs 的红色荧光几乎保持不变，而在 BSA 的敏化作用下，DC 的绿色荧光也出现了。BSA-AuNCs 对四环素抗生素中的 DC 表现出最高的响应，这归因于 BSA 对 DC 的最强敏化作用。BSA-AuNCs 还具有合成简单、响应时间短（1 分钟）和检测限低（36 nM）的特点。BSA-AuNCs 最终被应用于鱼类样品中 DC 的检测，并进一步制成便于携带的测试条。因此，本工作提出了一种设计用于在四环素抗生素中选择性检测 DC 的荧光探针的有效策略。
