Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani, 12120, Thailand.
Phytother Res. 2014 Nov;28(11):1671-5. doi: 10.1002/ptr.5180. Epub 2014 May 23.
Umbelliferone (UMB), a natural product of coumarin family, has been shown to reduce blood glucose and to improve lipid profiles in streptozotocin (STZ)-induced diabetic rats. Our objective was to examine the effect of UMB on adipogenesis by investigating its stimulatory effect on lipid accumulation and mRNA expression of adipogenic transcription factors and adipocyte-specific genes in 3 T3-L1 preadipocyte culture. An Oil Red O staining was used to monitor lipid accumulation, and we found that UMB treatment at concentration range of 10-100 μM significantly increased lipid accumulation of differentiating 3 T3-L1 cells. At the molecular level of adipogenesis, we examined the mRNA expression of adipogenic transcription factors, peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c. Those transcription factors were increased by UMB at 10-100 μM. Interestingly, UMB also stimulated the mRNA expression of adipocyte-specific genes, adipocyte fatty acid-binding protein, lipoprotein lipase, fatty acid synthase, fatty acid translocase, and adiponectin. Our findings indicate that the stimulatory effect of UMB on adipocyte differentiation likely occurs through up-regulation of adipogenic transcription factors and downstream adipocyte-specific gene expression.
Umbelliferone (UMB)，香豆素家族的一种天然产物，已被证明可降低链脲佐菌素 (STZ) 诱导的糖尿病大鼠的血糖并改善其血脂谱。我们的目的是通过研究 UMB 对脂肪生成的刺激作用，即其对 3T3-L1 前体脂肪细胞培养中脂肪细胞分化相关转录因子和脂肪细胞特异性基因的 mRNA 表达的影响，来检测 UMB 对脂肪生成的影响。油红 O 染色用于监测脂质积累，结果发现 UMB 在 10-100μM 的浓度范围内可显著增加分化的 3T3-L1 细胞的脂质积累。在脂肪生成的分子水平上，我们检测了脂肪生成转录因子过氧化物酶体增殖物激活受体 γ、CCAAT/增强子结合蛋白 α 和固醇调节元件结合蛋白 1c 的 mRNA 表达。这些转录因子在 10-100μM 的 UMB 作用下增加。有趣的是，UMB 还刺激了脂肪细胞特异性基因的 mRNA 表达，如脂肪细胞脂肪酸结合蛋白、脂蛋白脂肪酶、脂肪酸合成酶、脂肪酸转运蛋白和脂联素。我们的研究结果表明，UMB 对脂肪细胞分化的刺激作用可能是通过上调脂肪生成转录因子和下游脂肪细胞特异性基因的表达来实现的。
