Zou Bo, Ge Zhenzhen, Zhu Wei, Xu Ze, Li Chunmei
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Key Laboratory of Environment Correlative Food Science, Huazhong Agricultural University, Ministry of Education, Wuhan, China.
Eur J Nutr. 2015 Dec;54(8):1333-43. doi: 10.1007/s00394-014-0814-9. Epub 2014 Dec 16.
Currently, obesity has become a worldwide health problem. Adipocyte differentiation is closely associated with the onset of obesity. Our previous studies suggested that persimmon tannin might be a potent anti-adipogenic dietary bioactive compound. However, the mechanism of persimmon tannin on adipocyte differentiation is still unknown. The purpose of this study was to investigate the effect of persimmon tannin on adipogenic differentiation in 3T3-L1 preadipocytes and the underlying mechanisms.
Adipogenic differentiation was induced by cocktail in the presence or absence of persimmon tannin. Intracellular lipid accumulation was determined by Oil red O staining and enzymatic colorimetric methods. Gene expression and protein levels were measured by real time RT-PCR and Western blot.
Persimmon tannin inhibited intracellular lipid accumulation markedly, and the inhibitory effect was largely limited to the early stage of adipocyte differentiation. Persimmon tannin suppressed the expression of C/EBPα and peroxisome proliferator-activated receptor-γ (PPARγ), significantly. Furthermore, genes related to lipogenesis, such as sterol regulatory element-binding protein 1, were down-regulated by persimmon tannin. In addition, adipocyte fatty acid binding protein (aP2), which is a target gene of PPARγ, was suppressed by persimmon tannin notably. Correspondingly, the expression of miR-27a and miR-27b were up-regulated by persimmon tannin from Day 2 to Day 8 significantly.
Persimmon tannin inhibited adipocyte differentiation through regulation of PPARγ, C/EBPα and miR-27 in early stage of adipogenesis.
目前，肥胖已成为一个全球性的健康问题。脂肪细胞分化与肥胖的发生密切相关。我们之前的研究表明，柿子单宁可能是一种有效的抗脂肪生成的膳食生物活性化合物。然而，柿子单宁对脂肪细胞分化的机制仍不清楚。本研究的目的是探讨柿子单宁对3T3-L1前脂肪细胞脂肪生成分化的影响及其潜在机制。
在有或没有柿子单宁的情况下，用鸡尾酒诱导脂肪生成分化。通过油红O染色和酶比色法测定细胞内脂质积累。通过实时RT-PCR和蛋白质印迹法测量基因表达和蛋白质水平。
柿子单宁显著抑制细胞内脂质积累，且抑制作用主要局限于脂肪细胞分化的早期。柿子单宁显著抑制C/EBPα和过氧化物酶体增殖物激活受体-γ（PPARγ）的表达。此外，与脂肪生成相关的基因，如固醇调节元件结合蛋白1，被柿子单宁下调。此外，作为PPARγ靶基因的脂肪细胞脂肪酸结合蛋白（aP2）也被柿子单宁显著抑制。相应地，从第2天到第8天，柿子单宁显著上调miR-27a和miR-27b的表达。
柿子单宁在脂肪生成早期通过调节PPARγ、C/EBPα和miR-27抑制脂肪细胞分化。
