壳聚糖涂层可增强硫酸钙基抗生素疗法在慢性骨髓炎治疗中的疗效。
Chitosan coating to enhance the therapeutic efficacy of calcium sulfate-based antibiotic therapy in the treatment of chronic osteomyelitis.
作者信息
Beenken Karen E, Smith James K, Skinner Robert A, Mclaren Sandra G, Bellamy William, Gruenwald M Johannes, Spencer Horace J, Jennings Jessica A, Haggard Warren O, Smeltzer Mark S
机构信息
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Department of Biomedical Engineering, University of Memphis, Memphis, TN, USA.
出版信息
J Biomater Appl. 2014 Oct;29(4):514-23. doi: 10.1177/0885328214535452. Epub 2014 May 21.
We demonstrate that coating calcium sulfate with deacetylated chitosan enhances the elution profile of daptomycin by prolonging the period during which high concentrations of antibiotic are released. Coatings reduced initial bolus release of daptomycin by a factor of 10 to approximately 1000 µg/ml, and levels remained above 100 µg/ml for up to 10 days. Chitosan-coated and uncoated calcium sulfate implants with and without 15% daptomycin were evaluated in an experimental model of staphylococcal osteomyelitis through bacteriology scores, radiology, histopathology, and Gram staining. Significant reduction in bacteriology scores was observed for implants containing daptomycin and coated with chitosan compared with all the other groups. We confirm that the use of chitosan-coated calcium sulfate beads for local antibiotic delivery can be correlated with an improved therapeutic outcome following surgical debridement in the treatment of chronic osteomyelitis.
我们证明，用脱乙酰壳聚糖包覆硫酸钙可通过延长高浓度抗生素释放的时间来增强达托霉素的洗脱曲线。包覆层将达托霉素的初始大剂量释放降低了10倍，降至约1000µg/ml，且其水平在长达10天的时间内保持在100µg/ml以上。通过细菌学评分、放射学、组织病理学和革兰氏染色，在葡萄球菌性骨髓炎的实验模型中评估了含15%达托霉素和不含15%达托霉素的壳聚糖包覆和未包覆的硫酸钙植入物。与所有其他组相比，观察到含达托霉素且用壳聚糖包覆的植入物的细菌学评分显著降低。我们证实，在慢性骨髓炎的治疗中，使用壳聚糖包覆的硫酸钙珠进行局部抗生素递送与手术清创后改善的治疗结果相关。
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