Suñol M, Tovar J A, Aldazabal P, Torrado J
Cir Pediatr. 1989 Jan;2(1):13-8.
Aiming at depicting the advantages of chick embryo as a model of fetal surgery we report on our experience of 402 operations carried out in an attempt to induce fetal intestinal obstruction. We have used fertile domestic hen (gallus gallus) eggs incubated at 37.5 degrees with 80% humidity and turned every hour. At the 12th day of incubation we coagulated, after exposing the umbilical cord, a small bowel loop in the physiologic umbilical hernia through a 1 cm-wide opening in the shell which was then sealed with plastic sterile adhesive. After sacrifice at the 15th day of the 76 surviving embryos (19%) we found type I intestinal atresia with localized meconium peritonitis and dilated proximal loop in 46 cases. In 3 out of them there was, like in some human newborns with intestinal obstruction, increased amount of bile-stained amniotic fluid. Both macroscopic and microscopic findings were identical to those usually found in human atresia and in that experimentally induced in fetus of other animal species. We firmly believe that our model is as good as any other for this purpose and, at the same time, it is cheaper and easier to handle.
为了描述鸡胚作为胎儿手术模型的优势，我们报告了我们为诱导胎儿肠梗阻而进行的402例手术的经验。我们使用了在37.5摄氏度、湿度80%且每小时翻动一次的条件下孵化的受精家鸡蛋（原鸡）。在孵化的第12天，暴露脐带后，通过蛋壳上一个1厘米宽的开口，在生理性脐疝中凝固一小段肠袢，然后用无菌塑料粘合剂密封。在第15天对76个存活胚胎（19%）进行处死后，我们发现46例为I型肠闭锁，伴有局限性胎粪性腹膜炎和近端肠袢扩张。其中3例与一些患有肠梗阻的人类新生儿一样，羊水胆红素含量增加。宏观和微观发现与人类闭锁以及在其他动物物种胎儿中实验诱导的发现相同。我们坚信，就这个目的而言，我们的模型与任何其他模型一样好，同时，它更便宜且易于操作。
