Key Laboratory of Brain Research, Basic Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Key Laboratory of Brain Research, Basic Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Chin J Nat Med. 2014 May;12(5):382-92. doi: 10.1016/S1875-5364(14)60048-0.
To prepare high-purity ginseng total saponins from a water decoction of Chinese ginseng root.
Total saponins were efficiently purified by dynamic anion-cation exchange following the removal of hydrophilic impurities by macroporous resin D101. For quality control, ultrahigh-performance liquid chromatography with a charged aerosol detector (CAD) was applied to quantify marker components. The total saponin content was estimated by a colorimetric method using a vanillin-vitriol system and CAD response.
D201, which consisted of a cross-linked polystyrene matrix and -N(+)(CH3)3 functional groups, was the best of the four anion exchange resins tested. However, no significant difference in cation exchange ability was observed between D001 (strong acid) and D113 (weak acid), although they have different functional groups and matrices. After purification in combination with D101, D201, and D113, the estimated contents of total saponins were 107% and 90% according to the colorimetric method and CAD response, respectively. The total amount of representative ginsenosides Re, Rd, Rg1, and compound K was approximately 22% based on ultrahigh-performance liquid chromatography-CAD quantitative analysis.
These findings suggest that an ion exchange resin, combined with macroporous adsorption resin separation, is a promising and feasible purification procedure for neutral natural polar components.
从人参根水煎液中制备高纯度人参总皂苷。
通过大孔树脂 D101 去除亲水性杂质后，采用动态阴离子-阳离子交换高效纯化总皂苷。为了质量控制，应用带电气溶胶检测器（CAD）的超高效液相色谱法对标记成分进行定量。总皂苷含量通过香草醛-硫酸比色法和 CAD 响应进行估计。
在所测试的四种阴离子交换树脂中，D201 是最好的，它由交联聚苯乙烯基质和-N(+)(CH3)3 官能团组成。然而，D001（强酸）和 D113（弱酸）之间的阳离子交换能力没有明显差异，尽管它们具有不同的官能团和基质。经 D101、D201 和 D113 联合纯化后，根据比色法和 CAD 响应分别估计总皂苷含量为 107%和 90%。根据超高效液相色谱-CAD 定量分析，代表性人参皂苷 Re、Rd、Rg1 和化合物 K 的总量约为 22%。
这些发现表明，离子交换树脂与大孔吸附树脂分离相结合，是一种有前途且可行的中性天然极性成分的纯化方法。
