用高静水压和离子液体基双水相体系处理的纯化皂苷。
Purified Saponins in Treated with High Hydrostatic Pressure and Ionic Liquid-Based Aqueous Biphasic Systems.
作者信息
Ma Jing, Yang Hongkai, Chen Yajing, Feng Xiaoping, Wu Chunyu, Long Fangyu
机构信息
College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China.
出版信息
Foods. 2022 Jun 29;11(13):1930. doi: 10.3390/foods11131930.
L. (Cucurbitaceae) is rich in saponins, which have multiple biological effects. In this study, the total saponins of were extracted by high hydrostatic pressure (HHP) technology. The optimal extraction process was determined (ethanol concentration 68%, pressure-holding time 8 min, ratio of material to solvent 1:35 and pressure 510 MPa), and the extraction amount of saponins reached 127.890 mg/g. On this basis, an ionic liquid-based aqueous biphasic system was constructed to purify the total saponins. Under the optimized conditions, the purity of saponins was 76.06%. Liquid chromatography-mass spectrometry (LC/MS) was used to characterize the saponins in the purified extract of . It was found that there were four kinds of saponins in the extract of : kuguaglycoside A, momordicoside L, kuguacin B and kuguacin J, providing a basis for the study of the biological activity of saponins.
罗汉果（葫芦科）富含皂苷，具有多种生物学效应。在本研究中，采用高静水压（HHP）技术提取罗汉果总皂苷。确定了最佳提取工艺（乙醇浓度68%、保压时间8分钟、料液比1:35、压力510兆帕），皂苷提取量达到127.890毫克/克。在此基础上，构建了基于离子液体的双水相体系来纯化总皂苷。在优化条件下，罗汉果皂苷纯度为76.06%。采用液相色谱-质谱联用（LC/MS）对罗汉果纯化提取物中的皂苷进行表征。结果发现，罗汉果提取物中有四种皂苷：苦瓜苷A、罗汉果苷L、苦瓜素B和苦瓜素J，为皂苷生物活性研究提供了依据。
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