Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
Trends Biotechnol. 2014 Jul;32(7):381-8. doi: 10.1016/j.tibtech.2014.04.008. Epub 2014 May 21.
The ability to perturb individual cells and to obtain information at the single-cell level is of central importance for addressing numerous biological questions. Atomic force microscopy (AFM) offers great potential for this prospering field. Traditionally used as an imaging tool, more recent developments have extended the variety of cell-manipulation protocols. Fluidic force microscopy (FluidFM) combines AFM with microfluidics via microchanneled cantilevers with nano-sized apertures. The crucial element of the technology is the connection of the hollow cantilevers to a pressure controller, allowing their operation in liquid as force-controlled nanopipettes under optical control. Proof-of-concept studies demonstrated a broad spectrum of single-cell applications including isolation, deposition, adhesion and injection in a range of biological systems.
单细胞操纵和获取单细胞水平信息的能力对于解决众多生物学问题至关重要。原子力显微镜（AFM）为这个蓬勃发展的领域提供了巨大的潜力。传统上被用作成像工具，最近的发展扩展了各种细胞操作方案。流体力显微镜（FluidFM）通过带有纳米尺寸孔的微通道悬臂梁将 AFM 与微流控结合在一起。该技术的关键要素是将中空悬臂梁连接到压力控制器，使其能够在液体中作为力控制纳米管在光学控制下运行。概念验证研究表明，该技术在一系列生物系统中具有广泛的单细胞应用，包括分离、沉积、粘附和注射。
