Department of oncology, Nan Lou Division, Chinese PLA General Hospital, Beijing, P. R. China.
Department of Gastroenterology, Nan Lou Division, Chinese PLA General Hospital, Beijing, P. R. China.
PLoS One. 2014 May 23;9(5):e98041. doi: 10.1371/journal.pone.0098041. eCollection 2014.
In this study, we investigated a potential regulatory role of FBI-1 in transcription factor activity of ETS-1. The protein interaction was identified between ETS-1 and FBI-1 in lovo cells. The accumulating data showed that FBI-1 promoted the recruitment of ETS-1 to endogenous promoter of its target genes and increase ETS-1 accumulation in the nuclear. Our work also indicated that the FBI-1 enhances ETS-1 transcription factor activity via down-regulating p53-mediated inhibition on ETS-1. Further, FBI-1 plays a role in regulation of colorectal carcinoma cells proliferation. These findings supported that FBI-1 might be a potential molecule target for treating colorectal carcinoma.
在这项研究中，我们研究了 FBI-1 在 ETS-1 转录因子活性中的潜在调节作用。在 lovo 细胞中鉴定了 ETS-1 和 FBI-1 之间的蛋白相互作用。累积的数据表明，FBI-1 促进了 ETS-1 募集到其靶基因的内源性启动子，并增加了 ETS-1 在核内的积累。我们的工作还表明，FBI-1 通过下调 p53 对 ETS-1 的抑制作用增强了 ETS-1 转录因子的活性。此外，FBI-1 在调节结肠癌细胞增殖中发挥作用。这些发现支持 FBI-1 可能是治疗结直肠癌的潜在分子靶标。
