Dong Lun, Li Yuping, Han Chongxu, Wang Xiaodong, She Lei, Zhang Hengzhu
Department of Neurosurgery, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
Department of Central Laboratory, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
Int J Oncol. 2014 Aug;45(2):746-56. doi: 10.3892/ijo.2014.2459. Epub 2014 May 22.
MicroRNAs (miRNAs) are frequently dysregulated in glioblastoma (GBM) patients. It has been discovered that highly stable extracellular miRNAs circulate in the blood of both healthy individuals and patients. miRNAs in serum of patients with GBM and normal controls were analyzed by microarray analysis. The relevant bioinformatic analysis of the predicted target genes (gene ontology, pathway, gene network analysis) were performed. The miRNA microarray reveals differentially expressed miRNAs in serum between the GBM and normal controls. Of the 752 miRNAs, 115 miRNAs were upregulated in the GBM group, and 24 miRNAs were downregulated (fold change ≥2.0, P<0.01). By further analysis, we found that miR-576-5p, miR-340 and miR-626 were significantly overexpressed, but miR-320, let-7g-5p and miR-7-5P showed significantly low expression in GBM patients. By further bioinformatic analysis, we found that they possibly play important roles in the regulation of glioma signaling pathways. In summary, the six miRNAs are significant distinct in the peripheral blood of patients with GBM pathologies. These data suggest that the miRNA profile of the peripheral blood may serve as a new biomarker for glioma diagnosis with high specificity and sensitivity.
微小RNA（miRNA）在胶质母细胞瘤（GBM）患者中常常失调。已发现高度稳定的细胞外miRNA在健康个体和患者的血液中循环。通过微阵列分析对GBM患者血清和正常对照血清中的miRNA进行了分析。对预测的靶基因进行了相关生物信息学分析（基因本体论、通路、基因网络分析）。miRNA微阵列揭示了GBM组和正常对照组血清中差异表达的miRNA。在752个miRNA中，GBM组中有115个miRNA上调，24个miRNA下调（倍数变化≥2.0，P<0.01）。通过进一步分析，我们发现miR-576-5p、miR-340和miR-626显著过表达，但miR-320、let-7g-5p和miR-7-5P在GBM患者中表达显著降低。通过进一步的生物信息学分析，我们发现它们可能在胶质瘤信号通路的调控中发挥重要作用。总之，这六种miRNA在GBM病理患者的外周血中有显著差异。这些数据表明，外周血的miRNA谱可能作为一种具有高特异性和敏感性的胶质瘤诊断新生物标志物。
