Sharma S C, Singh B
School of Life Sciences, Guru Nanak Dev University, Amritsar, India.
Acta Biochim Pol. 1989;36(3-4):333-42.
Candida Kefyr cells and their spheroplasts grown in media enriched with choline, N,N'-dimethylethanolamine (DMEA) or ethanolamine (EA) showed decreased concanavalin A (Con A) and ph to hemagglutinin (PHA) mediated agglutination while supplementation with N-monomethylethanolamine (MMEA) increased PHA-mediated agglutination. In all cases, the amount of phospholipid was increased and, consequently, a decrease in the free sterol; phospholipid ratio was observed except in the case of MMEA where this ratio remained almost unchanged. In the cells grown in media enriched with choline. DMEA or MMEA, but not in the EA-supplemented medium, the phosphatidylcholine to phosphatidylethanolamine ratio was increased. Saturation of fatty acids as well as their chain length decreased, which could lead to increased membrane fluidity. No breakpoint in Arrhenius plots of Mg2+ -ATPase of the choline-supplmented cells was observed. Cells grown in media enriched with EA and MMEA did not show any sharp break in Arrhenius plots of Mg2+ -ATPase.
在富含胆碱、N，N'-二甲基乙醇胺（DMEA）或乙醇胺（EA）的培养基中生长的解脂假丝酵母细胞及其原生质球，其刀豆球蛋白A（伴刀豆球蛋白A，Con A）和植物血凝素（PHA）介导的凝集作用降低，而补充N-单甲基乙醇胺（MMEA）则增加PHA介导的凝集作用。在所有情况下，磷脂的量均增加，因此，游离甾醇；除MMEA的情况外，观察到磷脂比率降低，在MMEA的情况下该比率几乎保持不变。在富含胆碱、DMEA或MMEA的培养基中生长的细胞中，但在补充EA的培养基中则不然，磷脂酰胆碱与磷脂酰乙醇胺的比率增加。脂肪酸的饱和度及其链长降低，这可能导致膜流动性增加。在添加胆碱的细胞的Mg2 + -ATP酶的阿累尼乌斯图中未观察到断点。在富含EA和MMEA的培养基中生长的细胞在Mg2 + -ATP酶的阿累尼乌斯图中未显示任何明显的断点。
