Sharma S C, Singh B
Department of Biology, Guru Nanak Dev University, India.
Acta Microbiol Pol. 1988;37(2):175-82.
Supplementation of the growth medium with erosterol, cholesterol and lanosterol enriched the Candida kefyr cells, presumably cell membranes with sterols. Sterol enriched C. kefyr cells showed a decrease in percentage of PHA and Con-A mediated agglutination. Sterol supplementation also increased the sterol: phospholipid ratio and in such cells unsaturated fatty acids predominated over saturated ones. The overall effect of these changes resulted in rigidifying the cell membranes as indicated by shift of break in Arrhenius plots of Mg2+ ATPase. This showed that lectin mediated agglutination of yeast cells may be affected by its membrane fluidity.
用麦角固醇、胆固醇和羊毛甾醇补充生长培养基，可使高加索假丝酵母细胞富集，推测是细胞膜富含甾醇。富含甾醇的高加索假丝酵母细胞显示出PHA和伴刀豆球蛋白A介导的凝集百分比降低。甾醇补充还增加了甾醇与磷脂的比例，在这些细胞中不饱和脂肪酸占主导地位。这些变化的总体效果导致细胞膜僵化，这由Mg2+ATP酶的阿累尼乌斯图中转折点的变化表明。这表明凝集素介导的酵母细胞凝集可能受其膜流动性的影响。
