TU Dortmund University, Laboratory of Biochemical Engineering, Emil-Figge-Str. 66, 44227 Dortmund, Germany.
Bioresour Technol. 2013 May;136:102-8. doi: 10.1016/j.biortech.2013.02.046. Epub 2013 Feb 24.
Gas stripping and pervaporation are investigated for butanol recovery in a two-stage acetone-butanol-ethanol (ABE) fermentation process. The first stage is operated in a continuous mode and the second stage as a fed-batch. Gas stripping coupled to the second stage and operated intermittently enabled additional glucose feeding in the second stage and up to 59 g/L butanol and 73 g/L total ABE solvents in the condensate. Concentration of 167 g/L butanol and 269 g/L ABE in the permeate was measured in ex situ pervaporation experiments using a PDMS membrane at temperature of 37 °C and pressure of 10mbars. The "operating window" tool is introduced to evaluate the feasibility of the existing ABE fermentations operated as continuous with cell recycle, as two-stages, with biomass immobilization or with integrated product removal. This tool enables the identification of the most favorable process configuration, which is the combination of cell immobilization and integrated product removal.
汽提和渗透蒸发用于丙酮丁醇乙醇（ABE）发酵过程的两级中回收丁醇。第一级连续操作，第二级分批进料。与第二级间歇操作的汽提相结合，可在第二级进行额外的葡萄糖进料，并在冷凝物中获得高达 59 g/L 的丁醇和 73 g/L 的总 ABE 溶剂。在 37°C 和 10mbars 的压力下，使用 PDMS 膜在原位渗透蒸发实验中测量了渗透物中 167 g/L 的丁醇和 269 g/L 的 ABE。引入“操作窗口”工具来评估现有的 ABE 发酵作为连续操作与细胞回收、两级操作、生物量固定化或集成产物去除的可行性。该工具可用于确定最有利的工艺配置，即细胞固定化和集成产物去除的组合。
