Perullini Mercedes, Durrieu Claude, Jobbágy Matías, Bilmes Sara A
INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA, Buenos Aires, Argentina.
Laboratoire des Sciences de l'Environnement, Ecole Nationale des Travaux Publics de l'Etat, rue Maurice Audin, 69518 Vaulx-en-Velin, Cedex, France.
J Biotechnol. 2014 Aug 20;184:94-9. doi: 10.1016/j.jbiotec.2014.05.013. Epub 2014 May 23.
An advanced encapsulation matrix that efficiently protects microalgae from harmful UV light without causing toxicity to the entrapped culture is developed based on the electrostatic adsorption of the dye Rhodamine B on silica preformed particles during sol-gel synthesis. The three microalgae (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the Two-step procedure. Once entrapped in the silica gel, Rhodamine B act as an inner cut-off filter, protecting the encapsulated organisms from UV radiation. This matrix allows the sterilization of encapsulation devices without affecting the viability of the entrapped microalgae cells. The condensation of Si(IV) in the presence of silica particles with adsorbed dye generates silica matrices with good mechanical stability. Furthermore; no appreciable differences in microstructure, as assessed by SAXS (Small Angle X-ray Scattering), are caused by the addition of the dye.
基于溶胶 - 凝胶合成过程中罗丹明B染料在二氧化硅预制颗粒上的静电吸附作用，开发了一种先进的包封基质，该基质能有效保护微藻免受有害紫外线的伤害，同时不会对被包裹的培养物产生毒性。先前按照两步法将三种微藻（普通小球藻、斜生栅藻和莱茵衣藻）固定在藻酸盐中。一旦被包裹在硅胶中，罗丹明B就充当内部截止滤光器，保护被包裹的生物体免受紫外线辐射。这种基质允许对包封装置进行灭菌，而不会影响被包裹的微藻细胞的活力。在存在吸附有染料的二氧化硅颗粒的情况下，Si(IV) 的缩合产生具有良好机械稳定性的二氧化硅基质。此外，通过小角X射线散射（SAXS）评估，添加染料不会导致微观结构出现明显差异。
