Górak Monika, Żymańczyk-Duda Ewa
Department of Bioorganic Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
J Appl Phycol. 2017;29(1):245-253. doi: 10.1007/s10811-016-0952-y. Epub 2016 Sep 15.
This report, based on the previous studies, compares the reductive activity of different modes of following photobiocatalysts (on laboratory and preparative scale): , cf. and , toward diethyl esters of 2-oxopropylphosphonate (1), 2-oxo-2-phenylethylphosphonate (2), and 2-oxobutylphosphonate (3). It was confirmed that immobilization in alginate matrix do not affect the activity and viability of the biocatalysts. Corresponding ()-hydroxyphosphonates (1a-3a) were obtained with similar efficiency compared to the free-cell mode with the yield and of the optical purity respectively (e.g., experiments): (1) yield: 21 %, . 84 %; (2) yield 97 %, . 97; (3) yield 21 %, 89 %. Scaling up the processes for the best biocatalyst, , indicated that the use of free-living cells of cyanobacteria is more effective (640 mg of substrate 2, 44 % of yield, 91 % of ..), compared to the column bioreactor packed with immobilized cells of this photobiocatalyst (384 mg of substrate 2, 38 % of yield, 86 % of ). In the case of free and immobilized cells of cf. , agitation of the medium was the crucial activity mediator. Shaking culture of free cells of cf. converted the diethyl 2-oxo-2-phenylethylphosphonate (2) with the yield of 43 % (99 % of ) compared to 18 % (99 % of , stationary culture). Immobilized cells of this cyanobacterium were also more active toward (2) under shaking conditions (28 % of yield, 99 % of ) than free ones without agitation.
本报告基于先前的研究，比较了不同模式的光生物催化剂（在实验室规模和制备规模下）对2-氧代丙基膦酸二乙酯（1）、2-氧代-2-苯乙基膦酸酯（2）和2-氧代丁基膦酸酯（3）的还原活性：参见[相关文献]和[相关文献]。已证实，固定在藻酸盐基质中不会影响生物催化剂的活性和活力。与游离细胞模式相比，以相似的效率获得了相应的（）-羟基膦酸酯（1a - 3a），产率和光学纯度分别为（例如，实验）：（1）产率：21%，[光学纯度数值]84%；（2）产率97%，[光学纯度数值]97；（3）产率21%，[光学纯度数值]89%。对最佳生物催化剂[生物催化剂名称]进行工艺放大表明，与填充有该光生物催化剂固定化细胞的柱式生物反应器相比（384毫克底物2，产率38%，[光学纯度数值]86%），使用蓝藻的游离细胞更有效（640毫克底物2，产率44%，[光学纯度数值]91%）。在[生物催化剂名称]的游离细胞和固定化细胞的情况下，培养基的搅拌是关键的活性介质。与静止培养的18%（[光学纯度数值]99%）相比，[生物催化剂名称]游离细胞的振荡培养将2-氧代-2-苯乙基膦酸二乙酯（2）转化的产率为43%（[光学纯度数值]99%）。在振荡条件下，这种蓝藻的固定化细胞对（2）的活性也比未搅拌的游离细胞更高（产率28%，[光学纯度数值]99%）。
