Wu Yueting, Tang Lele, Huang Linhong, Han Zhizhong, Wang Jian, Pan Haibo
Fujian Key Lab of Medical Instrument & Pharmaceutical Technology, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
Institute of Research for Functional Materials, Yishan Campus, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Chemical Engineering, Qishan Campus, Fuzhou University, Fuzhou, Fujian 350108, China.
Mater Sci Eng C Mater Biol Appl. 2014 Jun 1;39:92-9. doi: 10.1016/j.msec.2014.02.012. Epub 2014 Feb 17.
In this study, we reported on a low detection limit penicillin biosensor with layer-by-layer (LbL) film containing single-graphene nanosheets (SGNs) preadsorbed with hematein, ionic liquids (ILs) and penicillinase. The penicillinase catalyzes the hydrolysis of penicillin to penicilloic acid, where H(+) is liberated and monitored amperometrically with hematein as a pH indicator. The SGN-hematein/ILs/penicillinase biosensor exhibited excellent performance for penicillin in PBS with a wide range from 1.25×10(-13) to 7.5×10(-3)M, and a low detection limit of 10(-13)M (0.04ppt, S/N≥3). Furthermore, the detection of penicillin concentration in real sample (milk) had acceptable accuracy with the assay system.
在本研究中，我们报道了一种低检测限的青霉素生物传感器，其采用层层（LbL）膜，该膜包含预先吸附苏木精、离子液体（ILs）和青霉素酶的单石墨烯纳米片（SGNs）。青霉素酶催化青霉素水解为青霉酸，在此过程中释放出H(+)，并以苏木精作为pH指示剂通过安培法进行监测。SGN-苏木精/ILs/青霉素酶生物传感器在磷酸盐缓冲盐水（PBS）中对青霉素表现出优异的性能，检测范围从1.25×10(-13)到7.5×10(-3)M，检测限低至10(-13)M（0.04ppt，S/N≥3）。此外，该检测系统对实际样品（牛奶）中青霉素浓度的检测具有可接受的准确度。
