Wang Hui, Ding Changwen, Du Haiping, Liu Hailun, Wang Yongli, Yu Deyue
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.
Biotechnol Lett. 2014 Sep;36(9):1881-92. doi: 10.1007/s10529-014-1549-7. Epub 2014 May 27.
MYC2 is a basic helix-loop-helix Leu zipper transcription factor (TF). Here, 22 putative soybean MYC-like TFs were identified bioinformatically. Of these TFs, seven MYC2-like genes without introns were isolated and characterized. All seven GmMYCs displayed transactivation activity in yeast cells. Six genes (excepting GmMYC3) were expressed in the roots, stems, leaves, flowers, and seed wall but not in the developing seeds and up-regulated after insect feeding. The GmMYC1 transgenic tobacco rejected common cutworm (CCW, Spodoptera litura Fabricius) more strongly and lost less leaf area than the control (2.94 ± 2.36 vs 7.84 ± 4.63 cm(2)). The average relative growth rate of CCW feeding on transgenic tobacco leaves was lower than on control tobacco leaves (136 ± 60 vs 271 ± 76 %). These results indicated that GmMYC could stimulate the defense mechanism against insects in plants.
MYC2是一种碱性螺旋-环-螺旋亮氨酸拉链转录因子（TF）。在此，通过生物信息学方法鉴定出22个假定的大豆MYC样转录因子。在这些转录因子中，分离并鉴定了7个无内含子的MYC2样基因。所有7个GmMYC在酵母细胞中均表现出反式激活活性。6个基因（GmMYC3除外）在根、茎、叶、花和种皮中表达，但在发育中的种子中不表达，且在昆虫取食后上调。与对照相比，转GmMYC1基因烟草对斜纹夜蛾（Spodoptera litura Fabricius）的抗性更强，叶片面积损失更少（2.94±2.36平方厘米对7.84±4.63平方厘米）。取食转基因烟草叶片的斜纹夜蛾的平均相对生长速率低于取食对照烟草叶片的斜纹夜蛾（136±60%对271±76%）。这些结果表明，GmMYC可刺激植物对昆虫的防御机制。
