Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou, China.
PLoS One. 2022 Oct 14;17(10):e0276075. doi: 10.1371/journal.pone.0276075. eCollection 2022.
Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as important molecular chaperones in a series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins are still unclear. Here, we showed that the promoter of OsCYP2 could function as a tissue-specific promoter by GUS staining. Moreover, we found that the promoter sequence contained not only core elements but also inducible elements. Then, the ABA-responsive element was used for cDNA library screening, and the transcription factor MYC2-like was identified by a yeast one-hybrid assay and confirmed through an electrophoretic mobility shift assay. Furthermore, the relative expression showed that MYC2-like was induced by abscisic acid. In addition, MYC2-like overexpression enhanced salt tolerance in transformants and partially restored the cyp2-RNAi line. In summary, we explored a novel transcriptional signal mediated by MYC2-like, a potential regulator of salt stress-related physiological processes in rice.
亲环蛋白属于肽基脯氨酰顺反异构酶，作为重要的分子伴侣在一系列生物学过程中发挥功能。然而，亲环蛋白的表达模式和信号转导途径仍不清楚。在这里，我们通过 GUS 染色显示 OsCYP2 的启动子可以作为组织特异性启动子发挥作用。此外，我们发现启动子序列不仅包含核心元件，还包含诱导元件。然后，我们使用 ABA 响应元件进行 cDNA 文库筛选，并通过酵母单杂交测定鉴定出转录因子 MYC2-like，随后通过电泳迁移率变动测定得到验证。此外，相对表达分析表明 MYC2-like 受到脱落酸的诱导。此外，MYC2-like 的过表达增强了转化体的耐盐性，并部分恢复了 cyp2-RNAi 系。总之，我们探索了由 MYC2-like 介导的一种新的转录信号，它可能是水稻中与盐胁迫相关的生理过程的调节剂。
