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敲低 VQ 基序蛋白编码基因 GmVQ58 增强大豆对斜纹夜蛾的抗性。

Knockdown of GmVQ58 encoding a VQ motif-containing protein enhances soybean resistance to the common cutworm (Spodoptera litura Fabricius).

机构信息

National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.

School of Life Sciences, Guangzhou University, Guangzhou, China.

出版信息

J Exp Bot. 2020 May 30;71(10):3198-3210. doi: 10.1093/jxb/eraa095.

DOI:10.1093/jxb/eraa095
PMID:32076725
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7475176/
Abstract

Plants have evolved complex defense mechanisms to withstand insect attack. Identification of plant endogenous insect resistance genes is of great significance for understanding plant-herbivore interactions and improving crop insect resistance. Soybean (Glycine max (L.) Merr.) is an important crop that is often attacked by the common cutworm (CCW) (Spodoptera litura Fabricius). In this study, based on our transcriptomic data, the gene GmVQ58, encoding a FxxxVQxxTG (VQ) motif-containing protein, was cloned and characterized. This gene showed the highest expression in the leaves and roots and was up-regulated significantly after CCW attack. Constitutive expression of GmVQ58 rescued the susceptibility of an Arabidopsis mutant to CCW, and interference of GmVQ58 in soybean hairy roots enhanced the resistance to CCW. Furthermore, GmVQ58 was localized to the nucleus and physically interacted with the transcription factor GmWRKY32. The expression of two defense-related genes, GmN:IFR and GmVSPβ, was up-regulated in GmVQ58-RNAi lines. Additionally, the promoter region of GmVQ58 was likely selected during domestication, resulting in different expression patterns in cultivated soybeans relative to wild soybeans. These results suggest that silencing GmVQ58 confers soybean resistance to CCW.

摘要

植物进化出了复杂的防御机制来抵御昆虫的攻击。鉴定植物内源性的昆虫抗性基因对于理解植物-草食动物相互作用和提高作物的昆虫抗性具有重要意义。大豆（Glycine max (L.) Merr.）是一种重要的作物，经常受到斜纹夜蛾（Spodoptera litura Fabricius）的攻击。在这项研究中，基于我们的转录组数据，克隆并表征了编码含有 FxxxVQxxTG（VQ）基序的蛋白的基因 GmVQ58。该基因在叶片和根部表达量最高，在受到斜纹夜蛾攻击后显著上调。GmVQ58 的组成型表达挽救了拟南芥突变体对斜纹夜蛾的敏感性，而在大豆毛状根中干扰 GmVQ58 则增强了对斜纹夜蛾的抗性。此外，GmVQ58 定位于细胞核，并与转录因子 GmWRKY32 发生物理相互作用。两个防御相关基因 GmN:IFR 和 GmVSPβ 在 GmVQ58-RNAi 系中上调表达。此外，GmVQ58 的启动子区域可能在驯化过程中受到选择，导致栽培大豆与野生大豆的表达模式不同。这些结果表明，沉默 GmVQ58 赋予了大豆对斜纹夜蛾的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/7475176/b7cb4459a145/eraa095f0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/7475176/14b724849667/eraa095f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/7475176/67f67a50c476/eraa095f0007.jpg
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