Nazir Nausheen, Khan Sajid, Karim Nasiara, Nisar Mohammad, Aziz Tariq, Shami Ashwag, Al-Asmari Fahad, Alhhazmi Areej A, Al-Joufi Fakhria A, Alwethaynani Maher S
Department of Biochemistry, University of Malakand, Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan.
Department of Pharmacy, University of Peshawar, Khyber Pakhtunkhwa, Peshawar, Pakistan.
Cell Biochem Biophys. 2025 May 1. doi: 10.1007/s12013-025-01767-6.
Elaeagnus umbellata, commonly known as autumn olive, is considered a medicinal plant of high value and belongs to the Elaeagnaceae family. It exhibits anti-ulcer, antimutagenic, antimicrobial, and neuroprotective properties. The leaves of E. umbellata reportedly have pharmacological activities, including antibacterial, anti-inflammatory, and anticancer effects. However, no in vivo studies have evaluated this plant's hepatoprotective potential. In this study, the hepatoprotective potential of E. umbellata was determined using an in vivo model. Extraction from the leaves of E. umbellata was carried out using standard methods, which then were subjected to gas chromatography and mass spectroscopy for characterization. Fourteen compounds were identified in the crude methanolic extract (Met-Ext) of E. umbellata leaves. Total phenolic and total flavonoid contents were assessed, and the extract was tested for hepatoprotective potential against carbon tetrachloride (CCL)-induced liver injury using rats as an experimental model. The samples exhibited antibacterial potential against bacterial strains such as Pseudomonas Aeruginosa 25619 (25 mm zone of inhibition) and Enterococcus faecalis 29212 (26 mm zone of inhibition). No inhibition was noted for Klebsiella pneumonia 43816 relative to the standard imipenem (34 mm zone of inhibition on average). A marked increase was observed in the levels of some serum liver biochemical parameters such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total cholesterol, serum creatinine, total serum bilirubin, total triglyceride, and low-density lipoprotein. By contrast, a reduction was observed in other parameters, such as high-density lipoprotein, in a group of animals treated with CCl. The extract possessed substantial protective properties against CCl-induced liver toxicity, thereby mitigating liver damage and restoring liver function. The results of this in vivo study indicate that the crude Met-Ext of E. umbellata leaves exhibits considerable hepatoprotective effects in a dose-dependent manner.
胡颓子，俗称秋橄榄，被认为是一种具有高价值的药用植物，属于胡颓子科。它具有抗溃疡、抗诱变、抗菌和神经保护特性。据报道，胡颓子的叶子具有药理活性，包括抗菌、抗炎和抗癌作用。然而，尚无体内研究评估这种植物的肝脏保护潜力。在本研究中，使用体内模型确定了胡颓子的肝脏保护潜力。采用标准方法从胡颓子叶中提取，然后进行气相色谱和质谱分析以进行表征。在胡颓子叶的粗甲醇提取物（Met-Ext）中鉴定出14种化合物。评估了总酚和总黄酮含量，并以大鼠为实验模型，测试了该提取物对四氯化碳（CCL）诱导的肝损伤的肝脏保护潜力。样品对铜绿假单胞菌25619（抑菌圈25毫米）和粪肠球菌29212（抑菌圈26毫米）等细菌菌株具有抗菌潜力。相对于标准亚胺培南（平均抑菌圈34毫米），肺炎克雷伯菌43816未观察到抑制作用。观察到一些血清肝脏生化参数水平显著升高，如天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、总胆固醇、血清肌酐、总血清胆红素、总甘油三酯和低密度脂蛋白。相比之下，在一组用CCl处理的动物中，其他参数如高密度脂蛋白有所降低。该提取物对CCl诱导的肝毒性具有显著的保护特性，并减轻了肝损伤并恢复了肝功能。这项体内研究的结果表明，胡颓子叶的粗甲醇提取物（Met-Ext）以剂量依赖的方式表现出相当大的肝脏保护作用。
