de Oliveira Jonatas Rafael, de Aguiar Almeida Rosilene Batista, das Graças Figueiredo Vilela Polyana, de Oliveira Felipe Eduardo, da Rocha Rosilene Fernandes, Jorge Antonio Olavo Cardoso, de Oliveira Luciane Dias
Laboratory of Microbiology and Immunology, Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista/UNESP, São José dos Campos, SP, Brazil.
Laboratory of Microbiology and Immunology, Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista/UNESP, São José dos Campos, SP, Brazil.
Arch Oral Biol. 2014 Aug;59(8):808-14. doi: 10.1016/j.archoralbio.2014.05.013. Epub 2014 May 13.
To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).
By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1β and TNF-α by ELISA.
The most effective concentration was 250 mg/mL and also promoted significant reduction (log₁₀) in the biofilms of S. aureus (0.438 ± 0.269), S. epidermidis (0.377 ± 0.298), S. mutans (0.244 ± 0.161) and C. albicans (0.746 ± 0.209). Cell viability was similar to 100%. The production of IL-1β was similar to the control group (p>0.05) and there was inhibition of TNF-α (p<0.01).
A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages.
评估牛蒡提取物对金黄色葡萄球菌、表皮葡萄球菌、变形链球菌、白色念珠菌、热带念珠菌和光滑念珠菌的抗菌活性。此外，分析该提取物对巨噬细胞（RAW 264.7）的细胞毒性。
采用肉汤微量稀释法，使用不同浓度的提取物（250 - 0.4 mg/mL）来确定浮游培养物中的最小杀菌浓度（MMC），并将最有效浓度用于丙烯酸树脂制成的圆盘上的生物膜。通过MTT法评估牛蒡提取物MMC对RAW 264.7的细胞毒性，并通过ELISA对IL - 1β和TNF - α进行定量分析。
最有效浓度为250 mg/mL，对金黄色葡萄球菌（0.438 ± 0.269）、表皮葡萄球菌（0.377 ± 0.298）、变形链球菌（0.244 ± 0.161）和白色念珠菌（0.746 ± 0.209）的生物膜也有显著减少（log₁₀）。细胞活力接近100%。IL - 1β的产生与对照组相似（p>0.05），且TNF - α受到抑制（p<0.01）。
牛蒡提取物对浮游培养物中的所有评估菌株具有杀菌作用，对生物膜具有抑菌作用，且对巨噬细胞无细胞毒性。
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