普通百里香提取物具有抗微生物和抗炎作用，且无细胞毒性和遗传毒性。

Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity.

机构信息

São Paulo State University (UNESP). Institute of Science and Technology. Department of Biosciences and Oral Diagnosis, São José dos Campos, SP, Brazil.

出版信息

Arch Oral Biol. 2017 Oct;82:271-279. doi: 10.1016/j.archoralbio.2017.06.031. Epub 2017 Jun 27.

DOI:10.1016/j.archoralbio.2017.06.031

PMID:28683409

Abstract

OBJECTIVES

This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity.

METHODS

Monomicrobial biofilms of Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48h and exposed for 5min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey's Test (P≤0.05).

RESULTS

All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells.

CONCLUSIONS

T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity.

摘要

目的

本研究评估了普通琉璃苣提取物的生物学效应，如对浮游培养物和单、多微生物生物膜的抗菌活性、细胞毒性、抗炎活性和遗传毒性。

方法

形成了白色念珠菌、金黄色葡萄球菌、粪肠球菌、变形链球菌和铜绿假单胞菌的单微生物生物膜以及白色念珠菌与每种细菌组成的多微生物生物膜，将植物提取物暴露于生物膜 5 分钟。还将植物提取物暴露于 RAW 264.7 小鼠巨噬细胞、FMM-1 人牙龈成纤维细胞、MCF-7 人乳腺癌细胞和 HeLa 宫颈癌细胞 5 分钟，通过 MTT、中性红（NR）和结晶紫（CV）测定分析细胞活力。24 小时后，用脂多糖（LPS）从大肠杆菌中定量检测 RAW 264.7 产生的白细胞介素 1β（IL-1β）和肿瘤坏死因子 α（TNF-α）。通过在 1000 个细胞中形成微核（MN）来评估植物提取物的遗传毒性。通过 T 检验或方差分析和 Tukey 检验（P≤0.05）对结果进行分析。