五味子中的二苯并环辛二烯木脂素及其对脂多糖激活的小胶质细胞中一氧化氮产生的抑制活性。

Dibenzocyclooctadiene lignans from Schisandra chinensis and their inhibitory activity on NO production in lipopolysaccharide-activated microglia cells.

作者信息

Hu Di, Yang Zhiyou, Yao Xuechun, Wang Hua, Han Na, Liu Zhihui, Wang Yu, Yang Jingyu, Yin Jun

机构信息

Development and Utilization Key Laboratory of Northeast Plant Materials of Liaoning Province, Department of Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Phytochemistry. 2014 Aug;104:72-8. doi: 10.1016/j.phytochem.2014.04.014. Epub 2014 May 23.

DOI:10.1016/j.phytochem.2014.04.014

PMID:24864016

Abstract

Four dibenzocyclooctadiene lignans, schisanchinins A-D, and 10 known compounds were isolated from the EtOAc extract of fruits of Schisandra chinensis (Turcz.) Baill. Structures of compounds 1-4 were elucidated using a combination of spectroscopic techniques, including MS, UV and IR, NMR ((1)H NMR, (13)C NMR, HMQC, HMBC). The stereochemistry of the chiral centers and the biphenyl configuration were determined using NOESY, as well as analysis of CD spectra. In vitro activity assays showed that 11 of the 14 compounds exhibited inhibitory activity on lipopolysaccharide (LPS)-induced NO release in primary murine BV2 microglia cells.

摘要

从五味子（Schisandra chinensis (Turcz.) Baill.）果实的乙酸乙酯提取物中分离得到了四种二苯并环辛二烯木脂素，即五味子素A-D，以及10种已知化合物。采用质谱（MS）、紫外光谱（UV）、红外光谱（IR）、核磁共振波谱（(1)H NMR、(13)C NMR、HMQC、HMBC）等多种光谱技术相结合的方法对化合物1-4的结构进行了鉴定。利用核Overhauser效应光谱（NOESY）以及圆二色光谱（CD）分析确定了手性中心的立体化学和联苯构型。体外活性试验表明，14种化合物中的11种对原代小鼠BV2小胶质细胞中脂多糖（LPS）诱导的一氧化氮（NO）释放具有抑制活性。