Kim Hye Mi, Kim Chul Young
College of Pharmacy and Institute of Pharmaceutical Sciences and Technology, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
Methods Mol Biol. 2025;2895:165-176. doi: 10.1007/978-1-0716-4350-1_12.
Lignans have garnered significant interest in various fields of pharmaceuticals, nutrition, and pesticides due to their diverse biological activities. This chapter primarily focuses on the extraction and purification methods of lignans from Schisandra, followed by an extensive examination of qualitative and quantitative analytical techniques, including thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Additionally, methods for structural analysis using nuclear magnetic resonance (NMR) are briefly summarized. The chapter also details approaches for determining the relative and absolute composition of lignan compounds, with illustrative examples provided.
由于木脂素具有多种生物活性，它们在制药、营养和农药等各个领域引起了极大的关注。本章主要关注五味子中木脂素的提取和纯化方法，随后对定性和定量分析技术进行了广泛研究，包括薄层色谱法（TLC）和高效液相色谱法（HPLC）。此外，还简要总结了使用核磁共振（NMR）进行结构分析的方法。本章还详细介绍了确定木脂素化合物相对和绝对组成的方法，并提供了示例。
