Jafernik Karolina, Kubica Paweł, Dziurka Michał, Kulinowski Łukasz, Korona-Głowniak Izabela, Elansary Hosam O, Waligórski Piotr, Skalicka-Woźniak Krystyna, Szopa Agnieszka
Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9 str., 30-688 Kraków, Poland.
Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21 str., 30-239 Kraków, Poland.
Pharmaceuticals (Basel). 2024 Mar 29;17(4):442. doi: 10.3390/ph17040442.
This research's scope encompassed biotechnological, phytochemical, and biological studies of , including investigations into its in vitro microshoot culture grown in PlantForm bioreactors (temporary immersion systems, TISs), as well as extracts from leaves of the parent plant, focusing on anti-inflammatory, antioxidant, anticancer, and antimicrobial activities. The phytochemical analysis included the isolation and quantification of 17 compounds from dibenzocyclooctadiene, aryltetralin lignans, and neolignans using centrifugal partition chromatography (CPC), HPLC-DAD, and UHPLC-MS/MS tandem mass spectrometry with triple quadrupole mass filter methods. Higher contents of compounds were found in microshoots extracts (max. 543.99 mg/100 g DW). The major compound was schisantherin B both in the extracts from microshoots and the leaves (390.16 and 361.24 mg/100 g DW, respectively). The results of the anti-inflammatory activity in terms of the inhibition of COX-1, COX-2, sPLA2, and LOX-15 enzymes indicated that PlantForm microshoot extracts showed strong activity against COX-1 and COX-2 (for 177 mg/mL the inhibition percentage was 76% and 66%, respectively). The antioxidant potential assessed using FRAP, CUPRAC, and DPPH assays showed that extracts from microshoot cultures had 5.6, 3.8, and 3.3 times higher power compared to extracts from the leaves of the parent plant, respectively. The total polyphenol content (TPC) was 4.1 times higher in extracts from the in vitro culture compared to the leaves. The antiproliferative activity against T-cell lymphoblast line Jurkat, breast adenocarcinoma cultures (MCF-7), colon adenocarcinoma (HT-29), and cervical adenocarcinoma (HeLa), showed that both extracts have considerable effects on the tested cell lines. The antimicrobial activity tested against strains of Gram-positive and Gram-negative bacteria and fungi showed the highest activity towards (MIC and MBC 0.625 mg/mL).
本研究的范围包括对[植物名称]的生物技术、植物化学和生物学研究，包括对其在PlantForm生物反应器（临时浸没系统，TISs）中生长的离体微芽培养物的研究，以及对母本植物叶片提取物的研究，重点关注抗炎、抗氧化、抗癌和抗菌活性。植物化学分析包括使用离心分配色谱法（CPC）、HPLC-DAD和带有三重四极杆质量过滤器的UHPLC-MS/MS串联质谱法从二苯并环辛二烯、芳基四氢萘木脂素和新木脂素中分离和定量17种化合物。在微芽提取物中发现了更高含量的化合物（最高543.99毫克/100克干重）。微芽提取物和叶片中的主要化合物均为五味子酯乙（分别为390.16和361.24毫克/100克干重）。就抑制COX-1、COX-2、sPLA2和LOX-15酶而言的抗炎活性结果表明，PlantForm微芽提取物对COX-1和COX-2具有较强活性（对于177毫克/毫升，抑制率分别为76%和66%）。使用FRAP、CUPRAC和DPPH测定法评估的抗氧化潜力表明，微芽培养物提取物的抗氧化能力分别比母本植物叶片提取物高5.6倍、3.8倍和3.3倍。体外培养物提取物中的总多酚含量（TPC）比叶片高4.1倍。对T细胞淋巴母细胞系Jurkat、乳腺腺癌培养物（MCF-7）、结肠腺癌（HT-29）和宫颈腺癌（HeLa）的抗增殖活性表明，两种提取物对受试细胞系均有显著影响。针对革兰氏阳性和革兰氏阴性细菌及真菌菌株测试的抗菌活性表明，对[具体菌株名称]的活性最高（MIC和MBC为0.625毫克/毫升）。
