Hayama Tadashi, Yoshida Hideyuki, Yamaguchi Masatoshi, Nohta Hitoshi
Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan.
Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan.
J Pharm Biomed Anal. 2014 Dec;101:151-60. doi: 10.1016/j.jpba.2014.04.035. Epub 2014 May 5.
Perfluoroalkyl-containing compounds have a unique 'fluorous' property that refers to the remarkably specific affinity they share. Fluorous compounds can be easily isolated from non-fluorous species on the perfluoroalkyl-functionalized stationary phases used in fluorous solid-phase extraction and fluorous liquid chromatography by means of fluorous-fluorous interactions (fluorophilicity). Recently, this unique specificity has been applied to the highly selective enrichment and analysis of different classes of biogenic and related compounds in complex samples. Because the biogenic compounds are generally not 'fluorous', they must be derivatized with appropriate perfluoroalkyl group-containing reagent in order to utilize fluorous interaction. In this review, we introduce the application of fluorous affinity techniques including derivatization methods to biogenic sample analysis.
含全氟烷基的化合物具有独特的“氟相”性质，这是指它们所共有的显著的特异性亲和力。通过氟相-氟相相互作用（亲氟性），在氟相固相萃取和氟相液相色谱中使用的全氟烷基官能化固定相上，氟相化合物可以很容易地与非氟相物质分离。最近，这种独特的特异性已应用于复杂样品中不同种类生物源及相关化合物的高选择性富集和分析。由于生物源化合物通常不是“氟相的”，为了利用氟相相互作用，它们必须用适当的含全氟烷基的试剂进行衍生化。在这篇综述中，我们介绍了包括衍生化方法在内的氟相亲和技术在生物源样品分析中的应用。
