Hayama Tadashi
Faculty of Pharmaceutical Sciences, Fukuoka University.
Yakugaku Zasshi. 2015;135(9):1043-8. doi: 10.1248/yakushi.15-00116.
A separation-oriented derivatization method combined with LC has been developed for the selective analysis of biogenic and related compounds. In this method, we utilized a specific affinity between perfluoroalkyl-containing compounds, i.e., 'fluorous' compounds (fluorophilicity). Our strategy involves the derivatization of target analytes with perfluoroalkyl reagents, followed by selective retention of the derivatives with a perfluoroalkyl-modified stationary phase LC column. The perfluoroalkylated derivatives are strongly retained on the column owing to their fluorophilicity, whereas non-derivatized species, such as sample matrices, are hardly retained. Therefore, utilizing this derivatization method, target analytes can be determined selectively without interference from matrices. This method has been successfully applied to the LC analysis of some biogenic and related compounds in complex biological samples.
已开发出一种与液相色谱相结合的分离导向衍生化方法，用于生物源及相关化合物的选择性分析。在该方法中，我们利用了含全氟烷基化合物（即“氟化物”化合物）之间的特定亲和力（亲氟性）。我们的策略包括用全氟烷基试剂对目标分析物进行衍生化，然后用全氟烷基改性固定相液相色谱柱对衍生物进行选择性保留。由于其亲氟性，全氟烷基化衍生物在柱上被强烈保留，而未衍生化的物质（如样品基质）几乎不被保留。因此，利用这种衍生化方法，可以在不受基质干扰的情况下选择性地测定目标分析物。该方法已成功应用于复杂生物样品中一些生物源及相关化合物的液相色谱分析。
