Abhijith K S, Sharma Richa, Ranjan Rajeev, Thakur M S
Fermentation Technology and Bioengineering Department, CSIR-Central Food Technological Research Institute, Mysore-570020, Karnataka, India.
Photochem Photobiol Sci. 2014 Jul;13(7):986-91. doi: 10.1039/c4pp00046c. Epub 2014 May 28.
In the present study we explored metal enhanced bioluminescence in luciferase enzymes for the first time. For this purpose a simple and reproducible one pot synthesis of gold-silver alloy nanoparticles was developed. By changing the molar ratio of tri-sodium citrate and silver nitrate we could synthesize spherical Au-Ag colloids of sizes ranging from 10 to 50 nm with a wide range of localized surface plasmon resonance (LSPR) peaks (450-550 nm). The optical tunability of the Au-Ag colloids enabled their effective use in enhancement of bioluminescence in a luminescent bacterium Photobacterium leiognathi and in luciferase enzyme systems from fireflies and bacteria. Enhancement of bioluminescence was 250% for bacterial cells, 95% for bacterial luciferase and 52% for firefly luciferase enzyme. The enhancement may be a result of energy transfer or plasmon induced enhancement. Such an increase can lead to higher sensitivity in detection of bioluminescent signals with potential applications in bio-analysis.
在本研究中，我们首次探索了金属增强荧光素酶中的生物发光现象。为此，我们开发了一种简单且可重复的一锅法合成金银合金纳米颗粒的方法。通过改变柠檬酸钠和硝酸银的摩尔比，我们能够合成尺寸范围为10至50纳米的球形金-银胶体，其具有广泛的局域表面等离子体共振（LSPR）峰（450 - 550纳米）。金-银胶体的光学可调性使其能够有效地增强发光细菌费氏弧菌以及萤火虫和细菌的荧光素酶系统中的生物发光。细菌细胞的生物发光增强了250%，细菌荧光素酶增强了95%，萤火虫荧光素酶增强了52%。这种增强可能是能量转移或等离子体诱导增强的结果。这种增加可以提高生物发光信号检测的灵敏度，并在生物分析中具有潜在应用。
