Kshirsagar Prakash G, De Matteis Valeria, Pal Sudipto, Sangaru Shiv Shankar
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Department of Mathematics and Physics "Ennio De Giorgi", University of Salento, 73100 Lecce, Italy.
Nanomaterials (Basel). 2023 Sep 1;13(17):2471. doi: 10.3390/nano13172471.
Facile synthesis of metal nanoparticles with controlled physicochemical properties using environment-friendly reagents can open new avenues in biomedical applications. Nanomaterials with controlled physicochemical properties have opened new prospects for a variety of applications. In the present study, we report a single-step photochemical synthesis of ~5 nm-sized silver (Ag) and gold (Au) nanoparticles (NPs), and Ag-Au alloy nanoparticles using L-tyrosine. The physicochemical and surface properties of both monometallic and bimetallic NPs were investigated by analytical, spectroscopic, and microscopic techniques. Our results also displayed an interaction between L-tyrosine and surface atoms that leads to the formation of AgAu NPs by preventing the growth and aggregation of the NPs. This method efficiently produced monodispersed NPs, with a narrow-sized distribution and good stability in an aqueous solution. The cytotoxicity assessment performed on breast cancer cell lines (MCF-7) revealed that the biofriendly L-tyrosine-capped AgNPs, AuNPs, and bimetallic AgAu NPs were biocompatible. Interestingly, AgAu NPs have also unveiled controlled cytotoxicity, cell viability, and in vitro peroxidase nanozyme activity reliant on metal composition and surface coating.
使用环境友好型试剂简便合成具有可控物理化学性质的金属纳米颗粒可为生物医学应用开辟新途径。具有可控物理化学性质的纳米材料为各种应用开启了新前景。在本研究中，我们报道了使用L-酪氨酸一步光化学合成约5纳米大小的银（Ag）和金（Au）纳米颗粒（NPs）以及Ag-Au合金纳米颗粒。通过分析、光谱和显微镜技术研究了单金属和双金属纳米颗粒的物理化学和表面性质。我们的结果还显示L-酪氨酸与表面原子之间存在相互作用，该相互作用通过阻止纳米颗粒的生长和聚集导致AgAu纳米颗粒的形成。此方法有效制备了单分散纳米颗粒，其粒径分布窄且在水溶液中具有良好的稳定性。对乳腺癌细胞系（MCF-7）进行的细胞毒性评估表明，生物友好的L-酪氨酸封端的AgNPs、AuNPs和双金属AgAu NPs具有生物相容性。有趣的是，AgAu NPs还显示出依赖于金属组成和表面涂层的可控细胞毒性、细胞活力和体外过氧化物酶纳米酶活性。
