Kilpeläinen Athina, Axelsson Robertson Rebecca, Leitner Thomas, Sandström Eric, Maeurer Markus, Wahren Britta
1 Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , Stockholm, Sweden .
AIDS Res Hum Retroviruses. 2014 Nov;30(11):1065-71. doi: 10.1089/AID.2013.0299. Epub 2014 Jul 16.
Using the early protein HIV Nef, new HLA class I binding epitopes of importance for immune responses to HIV were predicted for common African alleles. In total we identified 45 epitopes previously not described for the HLA alleles A30:01, A30:02, B58:01, and C07:01 and compared them to reported epitopes, primarily from HLA-A*02:01, from the Los Alamos database and our own vaccine studies. Related to its small size, the Nef gene/protein appears to be able to contribute effectively to confer both stronger and broader cellular immunogenicity to an HIV-1 vaccine. We also propose feasible mutations of such an additional vaccine antigen to preserve its immunogenicity, modified not to confer HLA or CD4(+) down-regulating activities. This article includes data on a valuable HIV immunogenic component for a vaccine in Africa.
利用早期蛋白HIV Nef，预测了对HIV免疫反应具有重要意义的新的HLA I类结合表位，针对常见的非洲等位基因。我们总共鉴定出45个以前未针对HLA等位基因A30:01、A30:02、B58:01和C07:01描述过的表位，并将它们与主要来自洛斯阿拉莫斯数据库和我们自己疫苗研究中的、报道的主要来自HLA-A*02:01的表位进行比较。鉴于其小尺寸，Nef基因/蛋白似乎能够有效地为HIV-1疫苗赋予更强和更广泛的细胞免疫原性。我们还提出了这种额外疫苗抗原的可行突变，以保留其免疫原性，同时进行修饰以不赋予HLA或CD4(+)下调活性。本文包含了非洲一种疫苗中一种有价值的HIV免疫原性成分的数据。
