在同时进行运动后，摄入蛋白质可增加肌原纤维蛋白的合成。
Protein ingestion increases myofibrillar protein synthesis after concurrent exercise.
作者信息
Camera Donny M, West Daniel W D, Phillips Stuart M, Rerecich Tracy, Stellingwerff Trent, Hawley John A, Coffey Vernon G
机构信息
1Exercise and Nutrition Research Group, School of Exercise Sciences, Australian Catholic University, Fitzroy, Victoria, AUSTRALIA; 2Exercise and Nutrition Research Group, School of Medical Sciences, Royal Melbourne Institute of Technology University, Melbourne, AUSTRALIA; 3Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario, CANADA; 4Nestlé Research Center, Nestec Ltd., Lausanne, SWITZERLAND; 5Canadian Sport Centre Pacific, Victoria, British Columbia, CANADA; 6Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UNITED KINGDOM; 7School of Exercise and Nutrition Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, AUSTRALIA.
出版信息
Med Sci Sports Exerc. 2015 Jan;47(1):82-91. doi: 10.1249/MSS.0000000000000390.
PURPOSE
We determined the effect of protein supplementation on anabolic signaling and rates of myofibrillar and mitochondrial protein synthesis after a single bout of concurrent training.
METHODS
Using a randomized crossover design, eight healthy males were assigned to experimental trials consisting of resistance exercise (8 × 5 leg extension, 80% 1RM) followed by cycling (30 min at approximately 70% V˙O2peak) with either postexercise protein (PRO, 25-g whey protein) or placebo (PLA) ingestion. Muscle biopsies were obtained at rest and at 1 and 4 h after exercise.
RESULTS
Akt and mTOR phosphorylation increased 1 h after exercise with PRO (175%-400%, P < 0.01) and was different from PLA (150%-300%, P < 0.001). Muscle RING finger 1 and atrogin-1 messenger RNA (mRNA) were elevated after exercise but were higher with PLA compared with those in PRO at 1 h (50%-315%, P < 0.05), whereas peroxisome proliferator-activated receptor gamma coactivator 1-alpha mRNA increased 4 h after exercise (620%-730%, P < 0.001), with no difference between treatments. Postexercise rates of myofibrillar protein synthesis increased above rest in both trials (75%-145%, P < 0.05) but were higher with PRO (67%, P < 0.05), whereas mitochondrial protein synthesis did not change from baseline.
CONCLUSIONS
Our results show that a concurrent training session promotes anabolic adaptive responses and increases metabolic/oxidative mRNA expression in the skeletal muscle. PRO ingestion after combined resistance and endurance exercise enhances myofibrillar protein synthesis and attenuates markers of muscle catabolism and thus is likely an important nutritional strategy to enhance adaptation responses with concurrent training.
目的
我们确定了单次联合训练后补充蛋白质对合成代谢信号以及肌原纤维和线粒体蛋白质合成速率的影响。
方法
采用随机交叉设计，将8名健康男性分配至实验试验组，试验包括阻力运动（8组，每组5次腿部伸展，80%的1次重复最大值），随后进行骑行（约70%最大摄氧量下持续30分钟），运动后分别摄入蛋白质（PRO，25克乳清蛋白）或安慰剂（PLA）。在静息状态以及运动后1小时和4小时采集肌肉活检样本。
结果
运动后1小时，摄入PRO组的Akt和mTOR磷酸化水平升高（175% - 400%，P < 0.01），且与摄入PLA组不同（150% - 300%，P < 0.001）。肌肉环形指蛋白1和atrogin - 1信使核糖核酸（mRNA）在运动后升高，但在运动后1小时，PLA组高于PRO组（50% - 315%，P < 0.05），而过氧化物酶体增殖物激活受体γ共激活因子1 - α mRNA在运动后4小时升高（620% - 730%，P < 0.001），两组间无差异。在两个试验中，运动后肌原纤维蛋白质合成速率均高于静息水平（75% - 145%，P < 0.05），但PRO组更高（67%，P < 0.05），而线粒体蛋白质合成与基线相比未发生变化。
结论
我们的结果表明，一次联合训练可促进合成代谢适应性反应，并增加骨骼肌中代谢/氧化mRNA的表达。在抗阻和耐力运动联合后摄入PRO可增强肌原纤维蛋白质合成，并减弱肌肉分解代谢标志物，因此可能是联合训练中增强适应性反应的重要营养策略。
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