睡前摄入蛋白质可增加耐力运动后夜间恢复期间的线粒体蛋白合成速率：一项随机对照试验。
Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial.
机构信息
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, P.O. Box 616, 6200 MD, Maastricht, The Netherlands.
FrieslandCampina, 3818 LE, Amersfoort, The Netherlands.
出版信息
Sports Med. 2023 Jul;53(7):1445-1455. doi: 10.1007/s40279-023-01822-3. Epub 2023 Mar 1.
BACKGROUND
Casein protein ingestion prior to sleep has been shown to increase myofibrillar protein synthesis rates during overnight sleep. It remains to be assessed whether pre-sleep protein ingestion can also increase mitochondrial protein synthesis rates. Though it has been suggested that casein protein may be preferred as a pre-sleep protein source, no study has compared the impact of pre-sleep whey versus casein ingestion on overnight muscle protein synthesis rates.
OBJECTIVE
We aimed to assess the impact of casein and whey protein ingestion prior to sleep on mitochondrial and myofibrillar protein synthesis rates during overnight recovery from a bout of endurance-type exercise.
METHODS
Thirty-six healthy young men performed a single bout of endurance-type exercise in the evening (19:45 h). Thirty minutes prior to sleep (23:30 h), participants ingested 45 g of casein protein, 45 g of whey protein, or a non-caloric placebo. Continuous intravenous L-[ring-C]-phenylalanine infusions were applied, with blood and muscle tissue samples being collected to assess overnight mitochondrial and myofibrillar protein synthesis rates.
RESULTS
Pooled protein ingestion resulted in greater mitochondrial (0.087 ± 0.020 vs 0.067 ± 0.016%·h, p = 0.005) and myofibrillar (0.060 ± 0.014 vs 0.047 ± 0.011%·h, p = 0.012) protein synthesis rates when compared with placebo. Casein and whey protein ingestion did not differ in their capacity to stimulate mitochondrial (0.082 ± 0.019 vs 0.092 ± 0.020%·h, p = 0.690) and myofibrillar (0.056 ± 0.009 vs 0.064 ± 0.018%·h, p = 0.440) protein synthesis rates.
CONCLUSIONS
Protein ingestion prior to sleep increases both mitochondrial and myofibrillar protein synthesis rates during overnight recovery from exercise. The overnight muscle protein synthetic response to whey and casein protein does not differ.
CLINICAL TRIAL REGISTRATION
NTR7251 .
背景
研究表明，睡前摄入酪蛋白可增加夜间睡眠时的肌原纤维蛋白合成速率。目前尚不清楚睡前摄入蛋白质是否也能增加线粒体蛋白合成速率。虽然有人认为酪蛋白可能是睡前蛋白质的首选来源，但尚无研究比较睡前摄入乳清蛋白与酪蛋白对夜间肌肉蛋白合成速率的影响。
目的
我们旨在评估睡前摄入酪蛋白和乳清蛋白对耐力型运动后夜间恢复期间线粒体和肌原纤维蛋白合成速率的影响。
方法
36 名健康年轻男性在晚上（19:45）进行了一次耐力型运动。在睡前 30 分钟（23:30），参与者摄入 45 克酪蛋白、45 克乳清蛋白或无热量安慰剂。应用连续静脉内 L-[ring-C]-苯丙氨酸输注，采集血液和肌肉组织样本以评估夜间线粒体和肌原纤维蛋白合成速率。
结果
混合蛋白摄入使线粒体（0.087±0.020 与 0.067±0.016%·h，p=0.005）和肌原纤维（0.060±0.014 与 0.047±0.011%·h，p=0.012）蛋白合成速率均显著高于安慰剂。酪蛋白和乳清蛋白摄入在刺激线粒体（0.082±0.019 与 0.092±0.020%·h，p=0.690）和肌原纤维（0.056±0.009 与 0.064±0.018%·h，p=0.440）蛋白合成速率方面无差异。
结论
睡前摄入蛋白质可增加运动后夜间恢复期间的线粒体和肌原纤维蛋白合成速率。乳清蛋白和酪蛋白对肌肉蛋白的夜间合成反应无差异。
临床试验注册号
NTR7251。
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