Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, the Netherlands.
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, the Netherlands; FrieslandCampina, 3818 LE Amersfoort, the Netherlands.
Cell Rep Med. 2023 Dec 19;4(12):101324. doi: 10.1016/j.xcrm.2023.101324.
The belief that the anabolic response to feeding during postexercise recovery is transient and has an upper limit and that excess amino acids are being oxidized lacks scientific proof. Using a comprehensive quadruple isotope tracer feeding-infusion approach, we show that the ingestion of 100 g protein results in a greater and more prolonged (>12 h) anabolic response when compared to the ingestion of 25 g protein. We demonstrate a dose-response increase in dietary-protein-derived plasma amino acid availability and subsequent incorporation into muscle protein. Ingestion of a large bolus of protein further increases whole-body protein net balance, mixed-muscle, myofibrillar, muscle connective, and plasma protein synthesis rates. Protein ingestion has a negligible impact on whole-body protein breakdown rates or amino acid oxidation rates. These findings demonstrate that the magnitude and duration of the anabolic response to protein ingestion is not restricted and has previously been underestimated in vivo in humans.
人们认为，运动后恢复期间进食引起的合成代谢反应是短暂的，存在上限，而且多余的氨基酸会被氧化，这种观点缺乏科学依据。我们采用全面的四重同位素示踪剂喂养输注方法，结果表明，与摄入 25 克蛋白质相比，摄入 100 克蛋白质会导致更大、更持久（>12 小时）的合成代谢反应。我们证明了膳食蛋白质衍生的血浆氨基酸可用性和随后掺入肌肉蛋白质中的剂量反应增加。摄入大量蛋白质还会进一步增加全身蛋白质净平衡、混合肌肉、肌原纤维、肌肉结缔组织和血浆蛋白质合成率。蛋白质摄入对全身蛋白质分解率或氨基酸氧化率几乎没有影响。这些发现表明，蛋白质摄入引起的合成代谢反应的幅度和持续时间不受限制，此前在人体中已经被低估。
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