Cho S Y, Kim S I, Kang S Y, Kong Y, Han S K, Shim Y S, Han Y C
Kisaengchunghak Chapchi. 1989 Mar;27(1):15-21. doi: 10.3347/kjp.1989.27.1.15.
To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels were measured by enzyme-linked immunosorbent assay (ELISA). All but one patient who needed retreatment became symptom-free within a week. Antibody levels were dropped near to or below a cut-off absorbance (abs.) of 0.25 in varying intervals from 4 to 18 months. Of 9 patients who were retested within 3 months, 5 revealed temporary elevation of antibody level. After the elevation, the levels began to decline slowly to negative ranges. If treated earlier after symptoms developed, the temporary elevation did not occur and intervals to negative conversion were shorter. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblot, antigen-antibody reactions in individual patient faded gradually without significant changes in reacting antigen bands.
为观察并殖吸虫病患者吡喹酮治疗后的抗体变化，收集了13例血清学诊断患者的46份血清样本，时间跨度为4至28个月。采用酶联免疫吸附测定（ELISA）检测特异性抗体（IgG）水平。除1例需要再次治疗的患者外，所有患者在一周内症状消失。抗体水平在4至18个月的不同时间段内降至接近或低于0.25的临界吸光度（abs.）。在3个月内重新检测的9例患者中，5例显示抗体水平暂时升高。升高后，抗体水平开始缓慢下降至阴性范围。如果在症状出现后尽早治疗，则不会出现暂时升高，且转阴间隔更短。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）/免疫印迹法，个体患者的抗原-抗体反应逐渐减弱，反应抗原条带无明显变化。
