Thiruvengadam M, Praveen N, Yu B R, Kim S H, Chung I M
Konkuk University Department of Applied Bioscience, College of Life and Environmental Science Seoul 143 701 South Korea.
Acta Biol Hung. 2014 Jun;65(2):144-55. doi: 10.1556/ABiol.65.2014.2.3.
Phenolic contents and antioxidant capacities from different solvent extracts (petroleum ether, ethyl acetate, methanol, butanol and water) of Aster scaber leaf were investigated. Antioxidant activity was evaluated by three different methods, namely DPPH radical scavenging activity, reducing power assay and phosphomolybdenum activity. A total of twenty-three polyphenolic compounds were identified and quantified from A. scaber leaf extracts, including hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other groups of phenolic compounds. Ultra high performance liquid chromatography (UHPLC) analysis of the leaf extract revealed that myricetin (4850.45 μg/g) was the most dominant flavonols, compared to quercetin and kaempferol. Caffeic acid was the dominant phenolic compound in A. scaber leaf extracts, it constituted about 104.20 μg/g, followed by gentisic acid (84.50 μg/g), gallic acid (61.05 μg/g) and homogentisic acid (55.65 μg/g). The total phenolic and flavonoid content was the highest in ethyl acetate extract (322.43 and 6.51 mg/g). The decreasing order of antioxidant activity among the A. scaber leaf extracts assayed through all the three methods was found to be ethyl acetate > butanol > methanol > petroleum ether > water extract.
对东风菜叶片不同溶剂提取物（石油醚、乙酸乙酯、甲醇、丁醇和水）中的酚类成分和抗氧化能力进行了研究。通过三种不同方法评估抗氧化活性，即DPPH自由基清除活性、还原能力测定和磷钼酸活性。从东风菜叶片提取物中总共鉴定并定量了23种多酚化合物，包括羟基苯甲酸、羟基肉桂酸、黄酮醇和其他酚类化合物组。叶片提取物的超高效液相色谱（UHPLC）分析表明，与槲皮素和山奈酚相比，杨梅素（4850.45μg/g）是最主要的黄酮醇。咖啡酸是东风菜叶片提取物中主要的酚类化合物，含量约为104.20μg/g，其次是龙胆酸（84.50μg/g）、没食子酸（61.05μg/g）和尿黑酸（55.65μg/g）。乙酸乙酯提取物中的总酚和黄酮含量最高（322.43和6.51mg/g）。通过所有三种方法测定的东风菜叶片提取物的抗氧化活性降低顺序为乙酸乙酯＞丁醇＞甲醇＞石油醚＞水提取物。
