Olah Neli-Kinga, Osser Gyongyi, Câmpean Ramona Flavia, Furtuna Flavia Roxana, Benedec Daniela, Filip Lorena, Raita Oana, Hanganu Daniela
Vasile Goldi Western University of Arad, Faculty of Pharmacy, Rebreanu Street, Arad, Romania / SC PlantExtrakt SRL, 407059, Rădaia, Cluj, Romania.
Vasile Goldi Western University of Arad, Faculty of Pharmacy, Rebreanu Street, Arad, Romania.
Pak J Pharm Sci. 2016 Nov;29(6 Suppl):2355-2361.
The purpose of this study was to compare the polyphenol profile of different Rosemary (Rosmarinus officinalis) extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied the polyphenols from hydroalcoholic extracts (1:5- dry plant:solvent) obtained from fresh respectively dry plant and the gemmotherapic extract obtained from fresh plant (1:20- dry plant: solvent). The polyphenol profile was evaluated by UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by UV-V is spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver nanoparticle (SNP) methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of total polyphenols, (0.601mg/ml rosmarinic acid), total flavonoids, (0.270mg/ml luteoline) and rosmarinic acid (0.350 mg/ml). The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by highest values of antioxidant activity: 39.1ml (DPPH), 7.7 ml (ABTS), 698mM ET/100 ml (FRAP), 1947 mM ET/100 ml (CUPRAC), 4570mM ET/100 ml (SNP). These differences in the polyphenols profiles show the importance of use the fresh plants for obtaining the good quality extracts.
本研究的目的是比较从干草药和新鲜草药中获得的不同迷迭香（迷迭香叶）提取物的多酚谱，并评估其抗氧化作用。研究了分别从新鲜植物和干燥植物中获得的水醇提取物（1:5 - 干植物:溶剂）中的多酚以及从新鲜植物中获得的宝石疗法提取物（1:20 - 干植物:溶剂）中的多酚。通过紫外 - 可见分光光度法、薄层色谱法和高效液相色谱法评估多酚谱。通过紫外 - 可见分光光度法测定总多酚和类黄酮的含量。通过DPPH、ABTS、FRAP、CUPRAC和银纳米颗粒（SNP）方法评估抗氧化作用。从新鲜植物中获得的水醇提取物含有最高浓度的总多酚（0.601mg/ml迷迭香酸）、总类黄酮（0.270mg/ml木犀草素）和迷迭香酸（0.350mg/ml）。宝石疗法提取物的浓度较低，但其提取率高于水醇提取物。上述水醇提取物中多酚的高含量通过以下抗氧化活性的最高值得到证实：39.1ml（DPPH）、7.7ml（ABTS）、698mM ET/100ml（FRAP）、1947mM ET/100ml（CUPRAC）、4570mM ET/100ml（SNP）。这些多酚谱的差异表明使用新鲜植物获取高质量提取物的重要性。
