Cole G M, Wu K, Timiras P S
Department of Physiology-Anatomy, University of California, Berkeley, CA 94720 U.S.A.
Int J Dev Neurosci. 1985;3(1):23-32. doi: 10.1016/0736-5748(85)90016-4.
Cloned human neuroblastoma cells have been induced to differentiate with retinoic acid whereupon they form an extensive network of processes and pseudoganglia. Ultrastructural examination shows these processes often contain assembled neurofilaments arranged parallel to microtubules in orderly arrangements characteristic of normal neurons. Perturbation of these differentiated, ganglion like cells with agents such as aluminum, zinc, and possibly leupeptin results in apparent neurofibrillary pathologies as evidenced by neurofilament specific immunofluorescent microscopy. A quantitative increase in neurofilament-specific antibody binding induced by aluminum and leupeptin were verified by flow cytometry with a fluorescence activated cell sorter. The utility of this system in screening agents for their capacity to produce neurofibrillary lesions characteristic of age-related human disorders is discussed.
克隆的人神经母细胞瘤细胞已被视黄酸诱导分化，随后形成广泛的突起网络和假神经节。超微结构检查显示，这些突起通常含有组装好的神经丝，它们与微管平行排列，呈正常神经元特有的有序排列。用铝、锌以及可能的亮抑酶肽等试剂干扰这些分化的、神经节样细胞，会导致明显的神经原纤维病理变化，这在神经丝特异性免疫荧光显微镜检查中得到了证实。通过荧光激活细胞分选仪进行的流式细胞术验证了铝和亮抑酶肽诱导的神经丝特异性抗体结合的定量增加。讨论了该系统在筛选具有产生与年龄相关的人类疾病特征性神经原纤维病变能力的试剂方面的实用性。
