Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152#, Luoyu Road, Wuhan, Hubei 430079, PR China.
Int J Biol Macromol. 2014 Aug;69:229-35. doi: 10.1016/j.ijbiomac.2014.05.038. Epub 2014 May 26.
(1→3)-β-d-glucan derived from Poria cocos hardly exhibits bioactivities. To extend its use, three types of (1→3)-β-d-glucan derivatives, which were sulfated (1→3)-β-d-glucan (S-P), carboxymethyl (1→3)-β-d-glucan (CMP) and carboxylmethyl (1→3)-β-d-glucan sulfate (S-CMP), were synthesized. Potential antioxidant activities of S-P, CMP and S-CMP were evaluated in vitro. The experiments of scavenging abilities of free radicals were carried out, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl. Deeply study of the derivatives' inhibitory effect for lipid peroxidation, DNA oxidative damage, erythrocyte hemolysis, and malondialdehyde (MDA) production were determined. And S-CMP significantly (P<0.01) increased the antioxidant activity of β-glucan. These results showed that multiple modifications of polysaccharides may bring the derivatives with excellent properties and various applications.
（1→3）-β-d-葡聚糖来源于茯苓，几乎没有生物活性。为了扩展其用途，合成了三种（1→3）-β-d-葡聚糖衍生物，即硫酸化（1→3）-β-d-葡聚糖（S-P）、羧甲基（1→3）-β-d-葡聚糖（CMP）和羧甲基（1→3）-β-d-葡聚糖硫酸酯（S-CMP）。体外评价了 S-P、CMP 和 S-CMP 的潜在抗氧化活性。进行了清除自由基能力的实验，如 1,1-二苯基-2-苦基肼（DPPH）、超氧阴离子和羟基。深入研究了衍生物对脂质过氧化、DNA 氧化损伤、红细胞溶血和丙二醛（MDA）生成的抑制作用。S-CMP 显著（P<0.01）提高了β-葡聚糖的抗氧化活性。这些结果表明，多糖的多种修饰可能使衍生物具有优异的性能和多种应用。
