Chu Shuang, Mao Xiao-Dong, Wang Li, Peng Wen
Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Nephrology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Evid Based Complement Alternat Med. 2016;2016:7272694. doi: 10.1155/2016/7272694. Epub 2016 Sep 20.
Vascular endothelial dysfunction can be induced by homocysteine (Hcy) through promoted oxidative stress. Huang Qi decoction (HQD) is a traditional Chinese medical formula and its components possess antioxidant effect. The study herein was therefore designed to investigate the effects of HQD at different dosage on endothelial dysfunction induced by Hcy. Tempol and apocynin were used to investigate whether antioxidant mechanisms were involved. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs) was determined by DHE staining. The assessment related to oxidative stress and NO bioavailability was performed by assay kits and western blot. In isometric tension experiment, HQD at the dose of 30 or 100 g/mL, tempol, or apocynin prevented impaired endothelium-dependent relaxation in isolated aortas elicited by Hcy. In cellular experiments, substantial enhancement in NADPH oxidase and ROS generation and reduction in NO bioavailability triggered by Hcy were reversed by pretreatment of HQD at the dose of 100 g/mL, tempol, or apocynin. The results proved that HQD at an appropriate dosage presented favorable effects on endothelial dysfunction initiated by Hcy through antioxidant mechanisms. HQD can act as a potent prescription for the treatment of endothelium related vascular complications.
同型半胱氨酸（Hcy）可通过促进氧化应激诱导血管内皮功能障碍。黄芪汤（HQD）是一种中药方剂，其成分具有抗氧化作用。因此，本研究旨在探讨不同剂量的HQD对Hcy诱导的内皮功能障碍的影响。使用Tempol和夹竹桃麻素研究是否涉及抗氧化机制。通过等长张力记录研究大鼠主动脉的内皮依赖性舒张。通过DHE染色测定人脐静脉内皮细胞（HUVECs）中的活性氧（ROS）。通过检测试剂盒和蛋白质免疫印迹法进行与氧化应激和一氧化氮生物利用度相关的评估。在等长张力实验中，30或100μg/mL剂量的HQD、Tempol或夹竹桃麻素可防止Hcy引起的离体主动脉内皮依赖性舒张受损。在细胞实验中，100μg/mL剂量的HQD、Tempol或夹竹桃麻素预处理可逆转Hcy引发的NADPH氧化酶和ROS生成的显著增加以及一氧化氮生物利用度的降低。结果证明，适当剂量的HQD通过抗氧化机制对Hcy引发的内皮功能障碍具有良好的作用。HQD可作为治疗内皮相关血管并发症的有效方剂。
