利用纯化的Cas9蛋白在果蝇中进行RNA引导的基因组编辑。
RNA-guided genome editing in Drosophila with the purified Cas9 protein.
作者信息
Lee Jeong-Soo, Kwak Su-Jin, Kim Jungeun, Kim Ae-Kyeong, Noh Hae Min, Kim Jin-Soo, Yu Kweon
机构信息
Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea Functional Genomics Department, Korea University of Science and Technology (KUST), Daejeon 305-333, Korea.
Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
出版信息
G3 (Bethesda). 2014 May 28;4(7):1291-5. doi: 10.1534/g3.114.012179.
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
我们报告了一种通过注射纯化的Cas9蛋白、tracrRNA和基因特异性crRNA复合物有效生成果蝇生殖系突变体的方法，该方法可能会减少由于Cas9蛋白的瞬时活性导致的延迟突变，并结合通过T7E1分析在GO创始人中进行简单的突变检测。
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