Dube Syamalima, Panebianco Lauren, Matoq Amr A, Chionuma Henry N, Denz Christopher R, Poiesz Bernard J, Dube Dipak K
Department of Medicine, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
Mol Biol Int. 2014;2014:896068. doi: 10.1155/2014/896068. Epub 2014 Apr 24.
We have investigated the expression of TPM1 α and TPM1 κ in mouse striated muscles. TPM1 α and TMP1 κ were amplified from the cDNA of mouse heart by using conventional RT-PCR. We have cloned the PCR amplified DNA and determined the nucleotide sequences. Deduced amino acid sequences show that there are three amino acid changes in mouse exon 2a when compared with the human TPM1 κ . However, the deduced amino acid sequences of human TPM1 α and mouse TPM1 α are identical. Conventional RT-PCR data as well as qRT-PCR data, calculating both absolute copy number and relative expression, revealed that the expression of TPM1 κ is significantly lower compared to TPM1 α in both mouse heart and skeletal muscle. It was also found that the expression level of TPM1 κ transcripts in mouse heart is higher than it is in skeletal muscle. To the best of our knowledge, this is the first report of the expression of TPM1 κ in mammalian skeletal muscle.
我们研究了TPM1α和TPM1κ在小鼠横纹肌中的表达情况。通过常规逆转录聚合酶链反应（RT-PCR）从小鼠心脏的cDNA中扩增出TPM1α和TPM1κ。我们克隆了PCR扩增的DNA并测定了核苷酸序列。推导的氨基酸序列显示，与人类TPM1κ相比，小鼠外显子2a中有三个氨基酸变化。然而，人类TPM1α和小鼠TPM1α的推导氨基酸序列是相同的。常规RT-PCR数据以及定量RT-PCR数据（计算绝对拷贝数和相对表达量）显示，在小鼠心脏和骨骼肌中，TPM1κ的表达与TPM1α相比显著较低。还发现小鼠心脏中TPM1κ转录本的表达水平高于骨骼肌。据我们所知，这是关于TPM1κ在哺乳动物骨骼肌中表达的首次报道。
