Merino Victor R C, Nakano Viviane, Finegold Sydney M, Avila-Campos Mario J
Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil.
Veterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USA ; Department of Medicine, School of Medicine, University of California, Los Angeles, CA, USA ; Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of California, Los Angeles, CA, USA.
Scientifica (Cairo). 2014;2014:594014. doi: 10.1155/2014/594014. Epub 2014 Apr 29.
The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB- and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.
采用TaqMan系统检测了腹泻患儿（110例）和非腹泻患儿（150例）粪便样本中艰难梭菌的16S rRNA基因以及编码毒素A（tcdA）、毒素B（tcdB）和二元毒素（cdtA/cdtB）的基因。260份粪便样本中有57份（21.9%）含有16S rRNA基因。在1份艰难梭菌阳性腹泻样本中验证了tcdA+/tcdB-和cdtA+/cdtB+的基因谱，在3份艰难梭菌阳性非腹泻样本中验证了tcdA+/tcdB+的基因谱。在无腹泻儿童的粪便中存在tcdA+/tcdB+，表明他们是产毒菌株的无症状携带者。
