Kang Su-Hwan, Kim Yeong-Su, Lee Hwan, Shin Kyung-Chul
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
R&D Center, Ildusanbang Co., Cheongsong 37434, Republic of Korea.
J Microbiol Biotechnol. 2025 Aug 7;35:e2505034. doi: 10.4014/jmb.2505.05034.
L., a medicinal herb used in East Asia, contains bioactive flavonoids such as sophoricoside and sophorabioside. Sophoricoside, a more bioavailable and therapeutically potent form, is known for its pharmacological properties, including anti-inflammatory, -cancer, and -osteoporotic effects. However, conversion of sophorabioside into sophoricoside has not been extensively studied. In this study, we aimed for the enzymatic conversion of sophorabioside, abundant in the fruits and small branches, into sophoricoside using α-L-rhamnosidase from . Optimal reaction conditions for this biotransformation were established, with maximal enzymatic activity observed at pH 6.0 and 55°C. Substrate specificity analysis revealed high selectivity of the enzyme for neohesperidose-containing flavonoids, including sophorabioside. Sophoricoside was efficiently produced from fruit and branch extracts, enhanced by 2.5% methanol. The biotransformed extracts demonstrated significant lipoxygenase inhibitory activity, suggesting enhanced anti-inflammatory potential, highlighting the potential of -derived sophoricoside-enriched products as functional food supplements with improved therapeutic properties.
L.是一种在东亚使用的药草，含有生物活性黄酮类化合物，如槐糖苷和槐二糖甙。槐糖苷是一种生物利用度更高、治疗效果更强的形式，以其药理特性而闻名，包括抗炎、抗癌和抗骨质疏松作用。然而，槐二糖甙向槐糖苷的转化尚未得到广泛研究。在本研究中，我们旨在利用来自[具体来源未提及]的α-L-鼠李糖苷酶将果实和小枝中丰富的槐二糖甙酶促转化为槐糖苷。确定了这种生物转化的最佳反应条件，在pH 6.0和55°C时观察到最大酶活性。底物特异性分析表明，该酶对含新橙皮糖的黄酮类化合物（包括槐二糖甙）具有高选择性。从果实和枝条提取物中高效生产出槐糖苷，2.5%的甲醇可增强其产量。生物转化提取物表现出显著的脂氧合酶抑制活性，表明抗炎潜力增强，突出了[具体来源未提及]来源的富含槐糖苷的产品作为具有改善治疗特性的功能性食品补充剂的潜力。
