Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
Int J Biol Macromol. 2014 Aug;69:252-9. doi: 10.1016/j.ijbiomac.2014.05.056. Epub 2014 May 28.
An alginate fraction, 04S2P, was isolated from the brown seaweed Sargassum fusiforme and was structurally characterized by the ratio (M/G) of β-d-mannuronic acid residues (M) to α-l-guluronic acid residues (G) via (1)H and (13)C NMR spectroscopy. When compared to commercial alginate (Alg) and alginates from other brown algae, 04S2P has a higher M/G ratio of 9.0:1.0 as determined by a modified high-performance liquid chromatography method after pre-column derivatization with PMP. Furthermore, the sulfated polysaccharides 04S2P-S and Alg-S were prepared by the chlorosulfonic acid-pyridine method. Both C-2 and/or C-3 of M and G residues of 04S2P-S were substituted by sulfate groups, with C-3 of M residues preferentially substituted. Their effects on tube formation of HMEC-1 cells were examined, and the results indicated that the sulfated Alg, Alg-S, exhibited a strong anti-angiogenic effect on HMEC-1 cells. The anti-tumor activity of native and sulfated alginates was tested on five different tumor cell lines. Alg-S demonstrated significant anti-tumor effects on the Bel7402, SMMC7721, and HT-29 cell lines, whereas 04S2P-S showed a distinct anti-tumor effect only on the Bel7402 cell line.
从褐藻马尾藻中分离得到一种褐藻胶级分 04S2P，通过（1）H 和（13）C NMR 光谱法，根据 β-d-甘露糖醛酸残基（M）与 α-l-古洛糖醛酸残基（G）的摩尔比（M/G）对其结构进行了表征。与商业褐藻胶（Alg）和其他褐藻来源的褐藻胶相比，04S2P 具有更高的 M/G 比，为 9.0:1.0，这是通过柱前衍生化 PMP 后改良的高效液相色谱法测定的。此外，通过氯磺酸-吡啶法制备了硫酸化多糖 04S2P-S 和 Alg-S。04S2P-S 的 M 和 G 残基的 C-2 和/或 C-3 被硫酸基团取代，C-3 优先取代 M 残基。研究了它们对 HMEC-1 细胞管形成的影响，结果表明，硫酸化 Alg 和 Alg-S 对 HMEC-1 细胞表现出强烈的抗血管生成作用。对天然和硫酸化褐藻胶的抗肿瘤活性在五种不同的肿瘤细胞系上进行了测试。Alg-S 对 Bel7402、SMMC7721 和 HT-29 细胞系表现出显著的抗肿瘤作用，而 04S2P-S 仅对 Bel7402 细胞系表现出明显的抗肿瘤作用。
