From the Laboratory of Biochemistry (Drs Alegre and González and Ms Rodriguez) and the Department of Medical Oncology (Drs Sanmamed and Martín-Algarra and Dr Carranza), University Clinic of Navarra, Pamplona, Navarra, Spain.
Arch Pathol Lab Med. 2014 Jun;138(6):828-32. doi: 10.5858/arpa.2013-0134-OA.
Malignant melanoma is an aggressive tumor that produces exosomes, which contain microRNAs (miRNAs) that could be of utility in following tumoral cell dysregulation. MicroR-125b is a miRNA whose down-regulation seems to be implicated in melanoma progression.
To analyze miR-125b levels in serum, and in exosomes obtained from serum, from patients with advanced melanoma.
Serum samples were obtained from 21 patients with advanced melanoma, from 16 disease-free patients with melanoma, and from 19 healthy volunteers. Exosomes were isolated from serum by precipitation, and miR-16 and miR-125b levels were quantified by real-time polymerase chain reaction.
MicroR-16, but not miR-125b, was detected in all samples, and miR-16 levels were significantly higher in serum than they were in exosomes. MicroR-16 expression levels did not differ significantly between the 2 groups (patients with melanoma and healthy donors). There was a significant relationship between miR-125b and miR-16 levels in exosomes. Additionally, miR-125b levels in exosomes were significantly lower in patients with melanoma compared with disease-free patients with melanoma and healthy controls.
Exosomes can provide a suitable material to measure circulating miRNA in melanoma, and miR-16 can be used as an endogenous normalizer. Lower levels of miR-125b in exosomes obtained from serum are associated with advanced melanoma disease, probably reflecting the tumoral cell dysregulation.
恶性黑色素瘤是一种侵袭性肿瘤，可产生含有 microRNAs（miRNAs）的外泌体，这些 miRNAs 可能对跟踪肿瘤细胞失调有用。miR-125b 是一种 miRNA，其下调似乎与黑色素瘤进展有关。
分析晚期黑色素瘤患者血清及血清来源外泌体中的 miR-125b 水平。
从 21 例晚期黑色素瘤患者、16 例无病期黑色素瘤患者和 19 例健康志愿者中采集血清样本。通过沉淀从血清中分离出外泌体，并通过实时聚合酶链反应定量检测 miR-16 和 miR-125b 的水平。
所有样本中均检测到 microR-16，但未检测到 miR-125b，且血清中 miR-16 水平明显高于外泌体。两组（黑色素瘤患者和健康供体）之间 miR-16 表达水平无显著差异。外泌体中 miR-16 和 miR-125b 水平之间存在显著相关性。此外，与无病期黑色素瘤患者和健康对照组相比，黑色素瘤患者外泌体中的 miR-125b 水平显著降低。
外泌体可以提供一种合适的材料来测量黑色素瘤中的循环 miRNA，并且 miR-16 可以用作内参。血清中外泌体中 miR-125b 水平较低与晚期黑色素瘤疾病相关，可能反映了肿瘤细胞失调。
