Zhang Zhen-Yu, Mai Yin, Yang Hao, Dong Pei-Yue, Zheng Xue-Li, Yang Gong-She
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, People's Republic of China.
Mol Cell Biochem. 2014 Oct;395(1-2):53-64. doi: 10.1007/s11010-014-2111-6. Epub 2014 May 31.
The process of preadipocytes differentiation plays a vital role in adipose tissue expansion and many factors are involved in this event. Cathepsin B (CTSB), secreted from lysosome, has been reported in regulating a variety of physiological processes. In this study, we demonstrated CTSB promotes lipid accumulation and adipogenic genes expression in porcine primary preadipocytes by degrading fibronectin (Fn), a key component of extracellular matrix. Lithium chloride (LiCl) is an activator of Wnt/β-catenin signaling through stabilizing β-catenin. We found that CTSB can relieve the anti-adipogenic effects of LiCl, indicating that CTSB could impact Wnt/β-catenin signaling pathway. Interestingly, Fn is an important target gene of Wnt/β-catenin. So we considered that CTSB promote preadipocytes differentiation by suppressing these two pathways.
前脂肪细胞分化过程在脂肪组织扩张中起着至关重要的作用，许多因素参与了这一过程。溶酶体分泌的组织蛋白酶B（CTSB）已被报道参与调节多种生理过程。在本研究中，我们证明CTSB通过降解细胞外基质的关键成分纤连蛋白（Fn）促进猪原代前脂肪细胞中的脂质积累和脂肪生成基因表达。氯化锂（LiCl）通过稳定β-连环蛋白来激活Wnt/β-连环蛋白信号通路。我们发现CTSB可以缓解LiCl的抗脂肪生成作用，表明CTSB可能影响Wnt/β-连环蛋白信号通路。有趣的是，Fn是Wnt/β-连环蛋白的一个重要靶基因。因此，我们认为CTSB通过抑制这两条途径促进前脂肪细胞分化。
