Yang So-Mi, Han So Hyun, Kim Bong-Gyu, Ahn Joong-Hoon
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 143-701, Korea.
J Ind Microbiol Biotechnol. 2014 Aug;41(8):1311-8. doi: 10.1007/s10295-014-1465-9. Epub 2014 May 31.
Flavonoids are ubiquitous phenolic compounds and at least 9,000 have been isolated from plants. Most flavonoids have been isolated and assessed in terms of their biological activities. Microorganisms such as Escherichia coli and Saccharomyces cerevisiae are efficient systems for the synthesis of flavonoids. Kaempferol 3-O-rhamnoside has notable biological activities such as the inhibition of the proliferation of breast cancer cells, the absorption of glucose in the intestines, and the inhibition of the self-assembly of beta amyloids. We attempted to synthesize kaempferol 3-O-rhamnoside from glucose in E. coli. Five flavonoid biosynthetic genes [tyrosine ammonia lyase (TAL), 4-coumaroyl CoA ligase (4CL), chalcone synthase (CHS), flavonol synthase (FLS), and flavonol 3-O-rhamnosyltransferase (UGT78D1)] from tyrosine were introduced into E. coli that was engineered to increase tyrosine production. By using this approach, the production of kaempferol 3-O-rhamnoside increased to 57 mg/L.
黄酮类化合物是普遍存在的酚类化合物，已从植物中分离出至少9000种。大多数黄酮类化合物已根据其生物活性进行分离和评估。诸如大肠杆菌和酿酒酵母等微生物是合成黄酮类化合物的有效系统。山奈酚3 - O - 鼠李糖苷具有显著的生物活性，如抑制乳腺癌细胞增殖、促进肠道对葡萄糖的吸收以及抑制β - 淀粉样蛋白的自组装。我们试图在大肠杆菌中从葡萄糖合成山奈酚3 - O - 鼠李糖苷。将来自酪氨酸的五个黄酮类生物合成基因[酪氨酸氨解酶（TAL）、4 - 香豆酰辅酶A连接酶（4CL）、查尔酮合酶（CHS）、黄酮醇合酶（FLS）和黄酮醇3 - O - 鼠李糖基转移酶（UGT78D1）]导入经过工程改造以增加酪氨酸产量的大肠杆菌中。通过这种方法，山奈酚3 - O - 鼠李糖苷的产量增加到了57毫克/升。
