Vucina I, Cisterna C, Moya R, Mejías E, Rojas C
Rev Med Chil. 1989 Feb;117(2):163-6.
Despite the recognized superiority of traditional culture methods to investigate bacteriuria their routine use in population studies is costly. We proposed to use Phase contrast microscopy (PCM) which allows clear visualization of bacteria with no centrifugation or staining. Urine samples from 214 patients were studied by PCM and results compared to those obtained by quantitative culture. MCF had 95% sensitivity and 96% specificity. Therefore, PCM may be used to screen urine samples for further bacteriologic study with the highest chance of obtaining positive cultures.
尽管传统培养方法在检测菌尿方面具有公认的优势，但在人群研究中常规使用成本高昂。我们建议使用相差显微镜（PCM），它无需离心或染色就能清晰观察到细菌。对214例患者的尿液样本进行了相差显微镜检查，并将结果与定量培养获得的结果进行比较。镜检法的敏感性为95%，特异性为96%。因此，相差显微镜可用于筛查尿液样本，以便进行进一步的细菌学研究，获得阳性培养结果的可能性最大。
